Leiferman K M, Norris P G, Murphy G M, Hawk J L, Winkelmann R K
Department of Dermatology, Mayo Clinic, Rochester, MN 55905.
J Am Acad Dermatol. 1989 Jul;21(1):75-80. doi: 10.1016/s0190-9622(89)70151-1.
Multiple sequential biopsy specimens of wheals elicited by a solar simulator in four patients with severe solar urticaria were studied with the use of indirect immunofluorescence for eosinophil granule major basic protein. Examination of control biopsy specimens from normal, unstimulated skin did not show eosinophils or major basic protein deposition. Five minutes after solar simulation, eosinophils were observed in vessels in the dermis, and 2 hours later there was marked tissue eosinophilia. Extracellular major basic protein was extensively deposited in the dermis at 2 and 24 hours, suggesting eosinophil degranulation. Thus evolution of the solar urticaria wheal is accompanied by infiltration of eosinophils and neutrophils and by tissue deposition of the eosinophil major basic protein, suggesting eosinophil degranulation.
对4例重度日光性荨麻疹患者经太阳模拟器激发产生的风团进行多次连续活检标本研究，采用间接免疫荧光法检测嗜酸性粒细胞颗粒主要碱性蛋白。对正常未受刺激皮肤的对照活检标本检查未发现嗜酸性粒细胞或主要碱性蛋白沉积。太阳模拟照射5分钟后，在真皮血管中观察到嗜酸性粒细胞，2小时后出现明显的组织嗜酸性粒细胞增多。2小时和24小时时，细胞外主要碱性蛋白在真皮中广泛沉积，提示嗜酸性粒细胞脱颗粒。因此，日光性荨麻疹风团的演变伴随着嗜酸性粒细胞和中性粒细胞浸润以及嗜酸性粒细胞主要碱性蛋白的组织沉积，提示嗜酸性粒细胞脱颗粒。
