Noguchi H, Kephart G M, Campbell R J, Li J T, Leiferman K M, Trocme S D, Gleich G J
Department of Immunology, Mayo Clinic, Rochester, MN 55905.
Ophthalmology. 1991 Jun;98(6):928-32. doi: 10.1016/s0161-6420(91)32200-0.
To investigate the participation of the eosinophil in orbital pseudotumor, the authors studied surgical biopsy specimens from nine patients with pseudotumor. Surgical biopsy specimens of orbital tissue from four patients with Graves' ophthalmopathy and autopsy specimens of orbital tissue from six patients without orbital diseases served as controls. Eosinophil infiltration and degranulation were assessed by immunofluorescence staining of formalin-fixed, paraffin-embedded tissues for the cytotoxic eosinophil granule major basic protein. Eosinophil infiltration and extracellular major basic protein deposition were evident in all orbital pseudotumor specimens. In contrast, no eosinophil infiltration or extracellular major basic protein deposition was present in any of the ten control specimens. These findings indicate that eosinophil degranulation is found in orbital pseudotumor, and they also identify yet another clinical entity where eosinophil infiltration and degranulation are associated with fibrosis.
为了研究嗜酸性粒细胞在眼眶假瘤中的作用，作者研究了9例假瘤患者的手术活检标本。选取4例格雷夫斯眼病患者的眼眶组织手术活检标本以及6例无眼眶疾病患者的眼眶组织尸检标本作为对照。通过对福尔马林固定、石蜡包埋组织进行免疫荧光染色，以检测细胞毒性嗜酸性粒细胞颗粒主要碱性蛋白，从而评估嗜酸性粒细胞浸润和脱颗粒情况。在所有眼眶假瘤标本中均可见嗜酸性粒细胞浸润和细胞外主要碱性蛋白沉积。相比之下，10例对照标本中均未发现嗜酸性粒细胞浸润或细胞外主要碱性蛋白沉积。这些发现表明眼眶假瘤中存在嗜酸性粒细胞脱颗粒现象，同时也确定了另一种嗜酸性粒细胞浸润和脱颗粒与纤维化相关的临床实体。
