Baĭkova L A, Fedorov V I, Cherkasova O P
Lab Delo. 1989(5):57-60.
A microcolumn variant of high-pressure liquid chromatography of human blood plasma corticosteroids using Milikhrom, a Soviet chromatograph, is suggested. The analysis involves hexane treatment of the plasma, chloroform extraction of corticosteroids, concentration of the extract, and chromatography in a 60 X 2 mm column packed with Silasorb sorbent, sph C18 (7.5 micros), methyl alcohol/water mixture (52:48) at a rate of 100 microliter/min and a detection wavelength 240 nm. The method permits an analysis of cortisone, hydrocortisone, corticosterone, and other corticosteroids with concentrations higher than 4 ng/ml.
提出了一种使用苏联产的米利色谱仪对人血浆皮质类固醇进行高压液相色谱分析的微柱变体方法。该分析包括用己烷处理血浆、用氯仿提取皮质类固醇、浓缩提取物，然后在填充有Silasorb吸附剂、sph C18（7.5微米）的60×2毫米柱中进行色谱分析，以甲醇/水混合物（52:48）为流动相，流速为100微升/分钟，检测波长为240纳米。该方法可分析可的松、氢化可的松、皮质酮和其他浓度高于4纳克/毫升的皮质类固醇。
