Ishii D, Hibi K, Asai K, Nagaya M, Mochizuki K, Mochida Y
J Chromatogr. 1978 Aug 21;156(1):173-80. doi: 10.1016/s0021-9673(00)83137-4.
The micro pre-column method, which is a useful method for the pre-treatment of samples, was applied to the analysis of corticosteroids in serum. Corticosteroids were concentrated in a micro pre-column packed with porous styrene-divinylbenzene copolymer particles and separated in a micro separation column packed with porous silica particles. Concentrations of corticosterone, cortisone and cortisol in the 20-130 ppb range were determined individually by pre-treatment of only 0.2 ml of serum.
微量预柱法是一种用于样品预处理的有效方法，已应用于血清中皮质类固醇的分析。皮质类固醇在填充有多孔苯乙烯 - 二乙烯基苯共聚物颗粒的微量预柱中富集，并在填充有多孔硅胶颗粒的微量分离柱中分离。通过仅对0.2 ml血清进行预处理，可分别测定20 - 130 ppb范围内的皮质酮、可的松和皮质醇的浓度。
