通过超声可切换近红外荧光实现深层组织中超越声学衍射极限的高分辨率成像。
High resolution imaging beyond the acoustic diffraction limit in deep tissue via ultrasound-switchable NIR fluorescence.
作者信息
Pei Yanbo, Wei Ming-Yuan, Cheng Bingbing, Liu Yuan, Xie Zhiwei, Nguyen Kytai, Yuan Baohong
机构信息
1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA [3].
1] Department of Bioengineering, The University of Texas at Arlington, Arlington, TX 76019, USA [2] Joint Biomedical Engineering Program, The University of Texas at Arlington and The University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.
出版信息
Sci Rep. 2014 Apr 15;4:4690. doi: 10.1038/srep04690.
Fluorescence imaging in deep tissue with high spatial resolution is highly desirable because it can provide details about tissue's structural, functional, and molecular information. Unfortunately, current fluorescence imaging techniques are limited either in penetration depth (microscopy) or spatial resolution (diffuse light based imaging) as a result of strong light scattering in deep tissue. To overcome this limitation, we developed an ultrasound-switchable fluorescence (USF) imaging technique whereby ultrasound was used to switch on/off the emission of near infrared (NIR) fluorophores. We synthesized and characterized unique NIR USF contrast agents. The excellent switching properties of these agents, combined with the sensitive USF imaging system developed in this study, enabled us to image fluorescent targets in deep tissue with spatial resolution beyond the acoustic diffraction limit.
具有高空间分辨率的深层组织荧光成像非常令人期待，因为它可以提供有关组织的结构、功能和分子信息的细节。不幸的是，由于深层组织中强烈的光散射，当前的荧光成像技术在穿透深度（显微镜）或空间分辨率（基于漫射光的成像）方面受到限制。为了克服这一限制，我们开发了一种超声可切换荧光（USF）成像技术，利用超声来开启/关闭近红外（NIR）荧光团的发射。我们合成并表征了独特的近红外超声可切换荧光造影剂。这些造影剂优异的切换特性，与本研究中开发的灵敏超声可切换荧光成像系统相结合，使我们能够在深层组织中对荧光靶点进行成像，其空间分辨率超过了声学衍射极限。
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