Yuan Baohong, Uchiyama Seiichi, Liu Yuan, Nguyen Kytai T, Alexandrakis George
Appl Phys Lett. 2012 Jul 16;101(3):33703. doi: 10.1063/1.4737211. Epub 2012 Jul 17.
The spatial resolution of fluorescence imaging techniques in deep optically turbid media such as tissues is limited by photon diffusion. To break the diffusion limit and achieve high-resolution and deep-tissue fluorescence imaging, a fundamentally different method was demonstrated based on a concept of ultrasound-switchable fluorescence. The results showed that a small fluorescent tube with a diameter of ∼180 μm at a depth of ∼20 mm in an optical scattering medium ([Formula: see text] and [Formula: see text] cm(-1)) can be clearly imaged with a size of ∼260 μm. The depth-to-resolution ratio is shown to be about one order of magnitude better than other deep-tissue fluorescence imaging techniques.
在诸如组织等深层光学浑浊介质中，荧光成像技术的空间分辨率受到光子扩散的限制。为了突破扩散极限并实现高分辨率的深层组织荧光成像，基于超声可切换荧光的概念展示了一种截然不同的方法。结果表明，在光学散射介质（[公式：见原文]和[公式：见原文]厘米⁻¹）中深度约为20毫米处，直径约为180微米的小型荧光管能够以约260微米的尺寸清晰成像。深度与分辨率之比显示比其他深层组织荧光成像技术大约好一个数量级。
