Uphoff Cord C, Drexler Hans G
Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Curr Protoc Mol Biol. 2014 Apr 14;106:28.5.1-28.5.12. doi: 10.1002/0471142727.mb2805s106.
Mycoplasma contaminations have a multitude of effects on cultured cell lines that may influence the results of experiments or pollute bioactive substances isolated from the eukaryotic cells. The elimination of mycoplasma contaminations from cell cultures with antibiotics has been proven to be a practical alternative to discarding and re-establishing important or irreplaceable cell lines. Different fluoroquinolones, tetracyclins, pleuromutilins, and macrolides shown to have strong anti-mycoplasma properties are employed for the decontamination. These antibiotics are applied as single treatments, as combination treatment of two antibiotics in parallel or successively, or in combination with a surface-active peptide to enhance the action of the antibiotic. The protocols in this unit allow eradication of mycoplasmas, prevention of the development of resistant mycoplasma strains, and potential cure of heavily contaminated and damaged cells. Consistent and permanent alterations to eukaryotic cells attributable to the treatment have not been demonstrated.
支原体污染对培养的细胞系有多种影响，可能会影响实验结果或污染从真核细胞中分离出的生物活性物质。事实证明，用抗生素消除细胞培养物中的支原体污染是丢弃并重新建立重要或不可替代的细胞系的一种切实可行的替代方法。已证明具有强大抗支原体特性的不同氟喹诺酮类、四环素类、截短侧耳素类和大环内酯类药物可用于去污。这些抗生素可单独使用、两种抗生素并行或先后联合使用，或与表面活性肽联合使用以增强抗生素的作用。本单元中的方案可根除支原体、预防耐药支原体菌株的产生，并可能治愈严重污染和受损的细胞。尚未证明该处理会导致真核细胞发生持续且永久性的改变。
