Patrick P Stephen, Kettunen Mikko I, Tee Sui-Seng, Rodrigues Tiago B, Serrao Eva, Timm Kerstin N, McGuire Sarah, Brindle Kevin M
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK; Department of Biochemistry, University of Cambridge, Cambridge, UK.
Magn Reson Med. 2015 Apr;73(4):1401-6. doi: 10.1002/mrm.25254. Epub 2014 Apr 14.
To assess the potential of a gene reporter system, based on a urea transporter (UTB) and hyperpolarized [(13) C]urea.
Mice were implanted subcutaneously with either unmodified control cells or otherwise identical cells expressing UTB. After injection of hyperpolarized [(13) C]urea, a spin echo sequence was used to measure urea concentration, T1 , and diffusion in control and UTB-expressing tissue.
The apparent diffusion coefficient of hyperpolarized urea was 21% lower in tissue expressing UTB, in comparison with control tissue (P < 0.05, 1-tailed t-test, n = 6 in each group). No difference in water apparent diffusion coefficient or cellularity between these tissues was found, indicating that they were otherwise similar in composition.
Expression of UTB, by mediating cell uptake of urea, lowers the apparent diffusion coefficient of hyperpolarized (13) C urea in tissue and thus the transporter has the potential to be used as a magnetic resonance-based gene reporter in vivo. Magn Reson Med 73:1401-1406, 2015. © 2014 Wiley Periodicals, Inc.
基于尿素转运体（UTB）和超极化[（13）C]尿素评估基因报告系统的潜力。
给小鼠皮下植入未修饰的对照细胞或表达UTB的相同细胞。注射超极化[（13）C]尿素后，使用自旋回波序列测量对照组织和表达UTB组织中的尿素浓度、T1以及扩散情况。
与对照组织相比，表达UTB的组织中超极化尿素的表观扩散系数低21%（P < 0.05，单尾t检验，每组n = 6）。未发现这些组织之间水的表观扩散系数或细胞密度存在差异，表明它们在组成上其他方面相似。
UTB的表达通过介导细胞对尿素的摄取，降低了组织中超极化（13）C尿素的表观扩散系数，因此该转运体有潜力用作基于磁共振的体内基因报告物。《磁共振医学》73:1401 - 1406，2015年。© 2014威利期刊公司
