超极化 [C] 尿素在小鼠肝脏的弥散加权成像。
Diffusion-weighted imaging of hyperpolarized [ C]urea in mouse liver.
机构信息
Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, California, USA.
Department of Pathology, University of California San Francisco, San Francisco, California, USA.
出版信息
J Magn Reson Imaging. 2018 Jan;47(1):141-151. doi: 10.1002/jmri.25721. Epub 2017 Apr 17.
PURPOSE
To compare the apparent diffusion coefficient (ADC) of hyperpolarized (HP) [ C, N]urea to the ADC of endogenous water in healthy and fibrotic mouse liver.
MATERIALS AND METHODS
ADC measurements for water and [ C]urea were made in agarose phantoms at 14.1T. Next, the ADC of water and injected HP [ C, N]urea were measured in eight CD1 mouse livers before and after induction of liver fibrosis using CCl . Liver fibrosis was quantified pathologically using the modified Brunt fibrosis score and compared to the measured ADC of water and urea.
RESULTS
In cell-free phantoms with 12.5% agarose, water ADC was nearly twice the ADC of urea (1.93 × 10 mm /s vs. 1.00 × 10 mm /s). The mean ADC values of water and [ C, N]urea in healthy mouse liver (±SD) were nearly identical [(0.75 ± 0.11) × 10 mm /s and (0.75 ± 0.22) × 10 mm /s, respectively]. Mean water and [ C, N]urea ADC values in fibrotic liver (±SD) were (0.84 ± 0.22) × 10 mm /s and (0.75 ± 0.15) × 10 mm /s, respectively. Neither water nor urea ADCs were statistically different in the fibrotic livers compared to baseline (P = 0.14 and P = 0.99, respectively). Water and urea ADCs were positively correlated at baseline (R = 0.52 and P = 0.045) but not in fibrotic livers (R = 0.23 and P = 0.23).
CONCLUSION
ADC of injected hyperpolarized urea in healthy liver reflects a smaller change as compared to free solution than ADC of water. This may reflect differences in cellular compartmentalization of the two compounds. No significant change in ADC of either water or urea were observed in relatively mild stages of liver fibrosis.
LEVEL OF EVIDENCE
1 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2018;47:141-151.
目的
比较超极化（HP）[C，N]尿素的表观扩散系数（ADC）与健康和纤维化小鼠肝内内源性水的 ADC。
材料与方法
在 14.1T 下，在琼脂糖模型中对水和[C]尿素进行 ADC 测量。接下来，在使用 CCl4 诱导肝纤维化之前和之后，在 8 只 CD1 小鼠肝脏中测量水和注射的 HP[C，N]尿素的 ADC。使用改良 Brunt 纤维化评分对肝纤维化进行病理量化，并与测量的水和尿素 ADC 进行比较。
结果
在 12.5%琼脂糖的无细胞模型中，水 ADC 几乎是尿素 ADC 的两倍（1.93×10 mm/s 与 1.00×10 mm/s）。健康小鼠肝脏中水和[C，N]尿素的平均 ADC 值（±SD）几乎相同[（0.75±0.11）×10 mm/s 和（0.75±0.22）×10 mm/s]。纤维化肝脏中水和[C，N]尿素的平均 ADC 值（±SD）分别为（0.84±0.22）×10 mm/s 和（0.75±0.15）×10 mm/s。与基线相比，纤维化肝脏中的水和尿素 ADC 均无统计学差异（P=0.14 和 P=0.99）。在基线时，水和尿素 ADC 呈正相关（R=0.52，P=0.045），但在纤维化肝脏中无相关性（R=0.23，P=0.23）。
结论
在健康肝脏中，与游离溶液相比，注射的超极化尿素的 ADC 变化较小。这可能反映了两种化合物在细胞区室化方面的差异。在相对轻度的肝纤维化阶段，观察到水和尿素 ADC 均无明显变化。
证据水平
1 技术功效：第 1 阶段 J. Magn. Reson. Imaging 2018;47:141-151.
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