Morii N, Nakao K, Matsumori A, Tomioka Y, Kawai C, Imura H
Department of Medicine, Kyoto University School of Medicine, Japan.
J Cardiovasc Pharmacol. 1989;13 Suppl 6:S5-8.
To elucidate the pathophysiological role of atrial natriuretic polypeptide (ANP) in diseased hearts, ANP levels in atrial and ventricular tissues and plasma were investigated in mice with congestive heart failure after viral myocarditis. The study was performed on day 14 after encephalomyocarditis viral inoculation, when congestive heart failure developed. The atrial ANP level increased to a value two times higher than that of noninoculated age-matched mice (the control mice). The ventricles of the control mice contained an approximately 1,000-fold lower ANP level than the atrial ANP level. The ventricular ANP concentration of the infected mice showed about a 21 times higher level than the control value. The plasma ANP level was also elevated 20 times. On immunofluorescence studies, increase of immunostaining were found in the atrial of the infected mice, and positive staining appeared on the cardiocytes in almost all areas of the remaining ventricular tissues of the infected mice, whereas staining was not found on the ventricles of the control mice. The staining seemed particularly strong in the area surrounding the myocardial lesions. These results indicate that atrial and ventricular ANP synthesis and ANP secretion increase in the heart with viral myocarditis. The marked increase in the ventricular ANP level suggests that the ventricle is another important production site for circulating ANP.
为阐明心房利钠多肽（ANP）在患病心脏中的病理生理作用，我们对病毒性心肌炎后发生充血性心力衰竭的小鼠的心房、心室组织及血浆中的ANP水平进行了研究。该研究在脑心肌炎病毒接种后第14天进行，此时充血性心力衰竭已经出现。心房ANP水平升至比未接种的年龄匹配小鼠（对照小鼠）高出两倍的值。对照小鼠心室中的ANP水平比心房ANP水平低约1000倍。感染小鼠的心室ANP浓度比对照值高出约21倍。血浆ANP水平也升高了20倍。在免疫荧光研究中，发现感染小鼠心房的免疫染色增加，并且在感染小鼠其余心室组织的几乎所有区域的心肌细胞上出现阳性染色，而对照小鼠的心室未发现染色。在心肌病变周围区域染色似乎特别强烈。这些结果表明，病毒性心肌炎时心脏中的心房和心室ANP合成及ANP分泌增加。心室ANP水平的显著升高表明心室是循环ANP的另一个重要产生部位。
