Parasannanavar Devaraj J, Rajadhyaksha Anjali, Ghosh Kanjaksha
Microbiology and Immunology Division, National Institute of Nutrition (ICMR), Jamai-Osmania, Tarnaka, Hyderabad, Andhra Pradesh 500007, India.
Department of Medicine, King Edward Memorial Hospital, Parel, Mumbai 400 012, India.
Autoimmune Dis. 2014;2014:327315. doi: 10.1155/2014/327315. Epub 2014 Mar 6.
Seronegative spondyloarthritis (SpA) are variably associated with HLA-B∗27 antigen. HLA-B∗27 negative SpA has also been reported from different parts of the world. There is paucity of data on this entity from Indian subcontinent. We studied 100 consecutively diagnosed HLA-B27 negative spondyloarthritis patients from a tertiary care center in India. Modified New York Criteria for ankylosing spondylitis (AS) and ESSG criteria for SpA were used for diagnosing patients. HLA-B∗27 typing was done by an in-house PCR-SSP technique in SpA patients to exclude B∗27 positive patients and PCR-SSOP technique was used to type 100 B∗27 negative SpA patients and 100 controls from the same ethnicity. Frequency of B∗07 was significantly increased (B∗07: % PF 54 versus 18; OR 5.348; 95% CI 2.808-10.186; P value 1.14E - 07), whereas frequency of B∗40 was significantly decreased (B∗40: % PF 17 versus 32; OR 0.435; 95% CI 0.222-0.850; P value 0.013) when compared with B∗27 negative controls. Among 100 SpA patients, 47 were undifferentiated spondyloarthritis and 33 patients were reactive arthritis patients. 40% of the patients were suffering from polyarticular arthritis, 35% had pauciarticular arthritis with knee joint, hip joint, ankle joint, and SI joint involvement. We conclude that B∗07 was significantly associated with B27 negative spondyloarthropathy from Western India and majority of B∗27 negative patients were uSpA.
血清阴性脊柱关节炎（SpA）与HLA - B∗27抗原存在不同程度的关联。世界各地均有HLA - B∗27阴性SpA的报道。来自印度次大陆的关于该实体的数据较少。我们研究了印度一家三级医疗中心连续诊断的100例HLA - B27阴性脊柱关节炎患者。采用改良纽约标准诊断强直性脊柱炎（AS），采用ESSG标准诊断SpA。通过内部PCR - SSP技术对SpA患者进行HLA - B∗27分型以排除B∗27阳性患者，并采用PCR - SSOP技术对100例B∗27阴性SpA患者和100例同种族对照进行分型。与B∗27阴性对照相比，B∗07的频率显著增加（B∗07：PF% 54对18；OR 5.348；95% CI 2.808 - 10.186；P值1.14E - 07），而B∗40的频率显著降低（B∗40：PF% 17对32；OR 0.435；95% CI 0.222 - 0.850；P值0.013）。在100例SpA患者中，47例为未分化脊柱关节炎，33例为反应性关节炎患者。40%的患者患有多关节关节炎，35%患有少关节关节炎，累及膝关节、髋关节、踝关节和骶髂关节。我们得出结论，B∗07与印度西部B27阴性脊柱关节病显著相关，且大多数B∗27阴性患者为未分化脊柱关节炎。
