Cruz Karla Dias, Cruz Thayana Araújo, Veras de Moraes Gabriela, Paredes-Santos Tatiana Christina, Attias Marcia, de Souza Wanderley
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil ; Instituto Nacional de Metrologia e Qualidade Industrial-Inmetro, 25250-020 Duque de Caxias, RJ, Brazil.
Biomed Res Int. 2014;2014:687835. doi: 10.1155/2014/687835. Epub 2014 Mar 9.
The intracellular parasite Toxoplasma gondii can penetrate any warm-blooded animal cell. Conserved molecular assemblies of host cell plasma membranes should be involved in the parasite-host cell recognition. Lipid rafts are well-conserved membrane microdomains that contain high concentrations of cholesterol, sphingolipids, glycosylphosphatidylinositol, GPI-anchored proteins, and dually acylated proteins such as members of the Src family of tyrosine kinases. Disturbing lipid rafts of mouse peritoneal macrophages and epithelial cells of the lineage LLC-MK2 with methyl-beta cyclodextrin (M β CD) and filipin, which interfere with cholesterol or lidocaine, significantly inhibited internalization of T. gondii in both cell types, although adhesion remained unaffected in macrophages and decreased only in LLC-MK2 cells. Scanning and transmission electron microscopy confirmed these observations. Results are discussed in terms of the original role of macrophages as professional phagocytes versus the LLC-MK2 cell lineage originated from kidney epithelial cells.
细胞内寄生虫刚地弓形虫能够穿透任何温血动物细胞。宿主细胞质膜保守的分子组装应参与寄生虫与宿主细胞的识别。脂筏是高度保守的膜微区，含有高浓度的胆固醇、鞘脂、糖基磷脂酰肌醇、糖基磷脂酰肌醇锚定蛋白以及双酰化蛋白，如酪氨酸激酶Src家族成员。用甲基-β-环糊精（MβCD）和制霉菌素干扰小鼠腹腔巨噬细胞和LLC-MK2谱系上皮细胞的脂筏，它们分别干扰胆固醇或利多卡因，显著抑制了两种细胞类型中弓形虫的内化，尽管巨噬细胞中的黏附不受影响，而在LLC-MK2细胞中仅有所降低。扫描电子显微镜和透射电子显微镜证实了这些观察结果。从巨噬细胞作为专职吞噬细胞的原始作用与源自肾上皮细胞的LLC-MK2细胞谱系的角度对结果进行了讨论。
