Kanda K, Sato H, Hashizume K, Yamada J
Department of Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Neurosci Lett. 1989 Apr 24;99(1-2):153-6. doi: 10.1016/0304-3940(89)90281-4.
The effect of blocking nerve conduction on retrograde horseradish peroxidase (HRP) labeling was investigated for rat hindlimb motoneurons. Conduction of the sciatic nerve in the left leg was blocked with tetrodotoxin, and HRP solution was injected into the medial gastrocnemius (MG) muscles of both sides. The total number of labeled MG motoneurons and the labeling intensities of individual cell somas were measured. There were no differences in these two parameters between the blocked and the intact sides. The results suggest that motoneurons are sufficiently well labeled with HRP, even if they are electrically silent.
研究了阻断神经传导对大鼠后肢运动神经元逆行辣根过氧化物酶（HRP）标记的影响。用河豚毒素阻断左腿坐骨神经的传导，并将HRP溶液注射到双侧腓肠肌内侧（MG）。测量标记的MG运动神经元总数和单个细胞体的标记强度。在阻断侧和完整侧之间，这两个参数没有差异。结果表明，即使运动神经元处于电静息状态，它们也能被HRP充分标记。
