Haase P, Hrycyshyn A W
Exp Neurol. 1986 Feb;91(2):399-403. doi: 10.1016/0014-4886(86)90079-8.
There is some evidence to suggest that the fascial sheaths of muscles are important in preventing the labeling of motoneurons that occurs, apparently, as a result of the diffusion of horseradish peroxidase (HRP) into the muscles. To test this possibility, Gelfoam soaked in HRP was implanted over flexor and extensor muscles in the proximal forelimb of the rat. When the fascial sheaths were damaged, labeled neurons were found in the motoneuronal pools of the exposed muscles; if intact, virtually no labeling of motoneurons was observed. These results suggest that, if intramuscular injections are to be used as a method for identifying motoneuronal pools, care should be taken when exposing the muscle to be injected, to ensure that surrounding muscles and their fascial sheaths are not damaged.
有证据表明，肌肉的筋膜鞘对于防止运动神经元被标记很重要，这种标记显然是由于辣根过氧化物酶（HRP）扩散到肌肉中而发生的。为了验证这种可能性，将浸泡过HRP的明胶海绵植入大鼠前肢近端的屈肌和伸肌上。当筋膜鞘受损时，在暴露肌肉的运动神经元池中发现了被标记的神经元；如果完整无损，则几乎观察不到运动神经元的标记。这些结果表明，如果要使用肌肉内注射作为识别运动神经元池的方法，在暴露待注射肌肉时应小心，以确保周围肌肉及其筋膜鞘不受损伤。
