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15S核糖体RNA高度保守的“530环”底部的一个不变核苷酸的替换导致酵母线粒体赭石型突变的抑制。

Substitution of an invariant nucleotide at the base of the highly conserved '530-loop' of 15S rRNA causes suppression of yeast mitochondrial ochre mutations.

作者信息

Shen Z H, Fox T D

机构信息

Section of Genetics and Development, Cornell University, Ithaca, NY 14853-2703.

出版信息

Nucleic Acids Res. 1989 Jun 26;17(12):4535-9. doi: 10.1093/nar/17.12.4535.

DOI:10.1093/nar/17.12.4535
PMID:2473436
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318012/
Abstract

We have determined the nucleotide sequence alteration in the 15S rRNA gene of a Saccharomyces cerevisiae strain carrying the previously described mitochondrial ochre suppressor, MSUI. The suppressor contains an A residue at position 633 of the yeast mitochondrial sequence, in place of the wild-type G. This position, located in the highly conserved region forming the stem of the '530-loop', corresponds to G517 of the Escherichia coli 16S rRNA and is occupied by G in all other known small rRNA sequences. This finding strongly supports the previous conclusions of others that the 530-loop region plays an important role in enhancing translational accuracy.

摘要

我们已经确定了携带先前描述的线粒体赭石抑制因子MSUI的酿酒酵母菌株15S rRNA基因中的核苷酸序列改变。该抑制因子在酵母线粒体序列的第633位含有一个A残基，取代了野生型的G。这个位置位于形成“530环”茎的高度保守区域，对应于大肠杆菌16S rRNA的G517，并且在所有其他已知的小rRNA序列中该位置均为G。这一发现有力地支持了其他人先前的结论，即530环区域在提高翻译准确性方面起着重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47cc/318012/e501fc271cb4/nar00129-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47cc/318012/e501fc271cb4/nar00129-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47cc/318012/e501fc271cb4/nar00129-0117-a.jpg

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本文引用的文献

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The molecular basis for rRNA-dependent spectinomycin resistance in Nicotiana chloroplasts.rRNA 依赖性壮观霉素抗性在烟草叶绿体中的分子基础。
EMBO J. 1987 Nov;6(11):3233-7. doi: 10.1002/j.1460-2075.1987.tb02640.x.
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Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide.酿酒酵母COX2 mRNA的线粒体翻译受指定前Cox2p前导肽的核苷酸序列控制。
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In vivo analysis of Saccharomyces cerevisiae COX2 mRNA 5'-untranslated leader functions in mitochondrial translation initiation and translational activation.酿酒酵母COX2 mRNA 5'-非翻译前导序列在线粒体翻译起始和翻译激活中的体内分析
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Pet127p, a membrane-associated protein involved in stability and processing of Saccharomyces cerevisiae mitochondrial RNAs.Pet127p，一种参与酿酒酵母线粒体RNA稳定性和加工的膜相关蛋白。
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