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抑制突变确定box9为酵母线粒体中内含子RNA高度有序结构的核心核苷酸序列。

Suppressor mutations identify box9 as a central nucleotide sequence in the highly ordered structure of intron RNA in yeast mitochondria.

作者信息

Holl J, Rödel G, Schweyen R J

出版信息

EMBO J. 1985 Aug;4(8):2081-5. doi: 10.1002/j.1460-2075.1985.tb03895.x.

DOI:10.1002/j.1460-2075.1985.tb03895.x
PMID:2415358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554465/
Abstract

Data presented here lend support to the notion that RNA splicing in yeast mitochondria depends on the formation of hybrid structures involving the well-conserved intron sequences box9 and box2. Starting with the cis-dominant splicing-defective box2 mutant G2590, a G----A transition, we isolated a revertant having a mitochondrial second site suppressor mutation, which restores splicing competence in the presence of the original mutation. Sequence analysis reveals that the suppressor mutation is a C----T transition in box9(5' part). This second mutation compensates for the first one in box2 and restores a box2/box9(5') hybrid. Combined with previous data demonstrating an interaction of the adjacent sequence box9(3' part) with the upstream box9c sequence in intron 4, the central role of box9 in the formation of the intron 4, the central role of box9 in the formation of the intron 4 RNA high order structure becomes evident.

摘要

此处呈现的数据支持了这样一种观点，即酵母线粒体中的RNA剪接依赖于涉及保守内含子序列box9和box2的杂交结构的形成。从顺式显性剪接缺陷型box2突变体G2590（一种G→A转换）开始，我们分离出了一个具有线粒体第二位点抑制突变的回复体，该突变在存在原始突变的情况下恢复了剪接能力。序列分析表明，抑制突变是box9（5'部分）中的一个C→T转换。这第二个突变补偿了box2中的第一个突变，并恢复了box2/box9（5'）杂交。结合先前的数据表明内含子4中相邻序列box9（3'部分）与上游box9c序列之间的相互作用，box9在形成内含子4的RNA高阶结构中的核心作用变得明显。

相似文献

1
Suppressor mutations identify box9 as a central nucleotide sequence in the highly ordered structure of intron RNA in yeast mitochondria.抑制突变确定box9为酵母线粒体中内含子RNA高度有序结构的核心核苷酸序列。
EMBO J. 1985 Aug;4(8):2081-5. doi: 10.1002/j.1460-2075.1985.tb03895.x.
2
Processing of yeast mitochondrial RNA: involvement of intramolecular hybrids in splicing of cob intron 4 RNA by mutation and reversion.酵母线粒体RNA的加工：通过突变和回复突变研究分子内杂交体在细胞色素b氧化酶亚基1内含子4 RNA剪接中的作用
Cell. 1983 May;33(1):195-202. doi: 10.1016/0092-8674(83)90348-3.
3
Substitution of an invariant nucleotide at the base of the highly conserved '530-loop' of 15S rRNA causes suppression of yeast mitochondrial ochre mutations.15S核糖体RNA高度保守的“530环”底部的一个不变核苷酸的替换导致酵母线粒体赭石型突变的抑制。
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Nuclear suppression of a mitochondrial RNA splice defect: nucleotide sequence and disruption of the MRS3 gene.线粒体RNA剪接缺陷的核抑制：MRS3基因的核苷酸序列及破坏
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Critical sequences within mitochondrial introns: pleiotropic mRNA maturase and cis-dominant signals of the box intron controlling reductase and oxidase.线粒体内含子中的关键序列：多效性mRNA成熟酶以及控制还原酶和氧化酶的盒式内含子的顺式显性信号。
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7
Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number.当以高拷贝数存在时，三个核基因可抑制酵母线粒体剪接缺陷。
Curr Genet. 1987;12(7):503-9. doi: 10.1007/BF00419559.
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MRS3 and MRS4, two suppressors of mtRNA splicing defects in yeast, are new members of the mitochondrial carrier family.MRS3和MRS4是酵母中线粒体RNA剪接缺陷的两个抑制因子，它们是线粒体载体家族的新成员。
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Mol Cell Biol. 2003 Dec;23(23):8809-19. doi: 10.1128/MCB.23.23.8809-8819.2003.
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Determination of functional domains in intron bI1 of yeast mitochondrial RNA by studies of mitochondrial mutations and a nuclear suppressor.通过线粒体突变和核抑制因子研究确定酵母线粒体RNA内含子bI1中的功能结构域
EMBO J. 1983;2(11):2047-52. doi: 10.1002/j.1460-2075.1983.tb01698.x.

引用本文的文献

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A single mutation in the 15S rRNA gene confers non sense suppressor activity and interacts with mRF1 the release factor in yeast mitochondria.15S核糖体RNA基因中的单个突变赋予无义抑制活性，并与酵母线粒体中的释放因子mRF1相互作用。
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Selection of cryptic 5' splice sites by group II intron RNAs in vitro.II类内含子RNA在体外对隐蔽5'剪接位点的选择
Nucleic Acids Res. 1988 Aug 11;16(15):7383-95. doi: 10.1093/nar/16.15.7383.
3
Three class I introns in the ND4L/ND5 transcriptional unit of Neurospora crassa mitochondria.粗糙脉孢菌线粒体ND4L/ND5转录单元中的三个I类内含子。
Mol Gen Genet. 1987 Feb;206(2):318-25. doi: 10.1007/BF00333590.
4
Compensatory mutations demonstrate that P8 and P6 are RNA secondary structure elements important for processing of a group I intron.补偿性突变表明，P8和P6是对I组内含子加工很重要的RNA二级结构元件。
Nucleic Acids Res. 1989 Jan 25;17(2):675-89. doi: 10.1093/nar/17.2.675.
5
RNA splicing in yeast mitochondria: DNA sequence analysis of mit- mutants deficient in the excision of introns aI1 and aI2 of the gene for subunit I of cytochrome c oxidase.酵母线粒体中的RNA剪接：细胞色素c氧化酶亚基I基因的内含子aI1和aI2切除缺陷的mit-突变体的DNA序列分析
Curr Genet. 1988 Mar;13(3):219-26. doi: 10.1007/BF00387767.
6
Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number.当以高拷贝数存在时，三个核基因可抑制酵母线粒体剪接缺陷。
Curr Genet. 1987;12(7):503-9. doi: 10.1007/BF00419559.

本文引用的文献

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Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.两个内含子家族中RNA二级结构的保守性，包括线粒体、叶绿体和核编码成员。
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Cell. 1982 Jun;29(2):527-36. doi: 10.1016/0092-8674(82)90169-6.
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Cell. 1982 Jun;29(2):517-26. doi: 10.1016/0092-8674(82)90168-4.
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Expression of the "split gene" COB in yeast mtDNA. Translation of intervening sequences in mutant strains.酵母线粒体DNA中“断裂基因”COB的表达。突变菌株中介入序列的翻译。
J Biol Chem. 1981 Apr 10;256(7):3525-31.
5
Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.细胞色素b野生型和box3突变体中内含子及侧翼外显子的序列揭示了一个由内含子编码的交错剪接蛋白。
Cell. 1980 Nov;22(2 Pt 2):333-48. doi: 10.1016/0092-8674(80)90344-x.
6
Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.真菌线粒体内含子的比较揭示了RNA二级结构中广泛的同源性。
Biochimie. 1982 Oct;64(10):867-81. doi: 10.1016/s0300-9084(82)80349-0.
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Making ends meet: a model for RNA splicing in fungal mitochondria.收支平衡：真菌线粒体中RNA剪接的一种模型。
Nature. 1982 Dec 23;300(5894):719-24. doi: 10.1038/300719a0.
8
Functional domains in introns: trans-acting and cis-acting regions of intron 4 of the cob gene.内含子中的功能结构域：cob基因第4内含子的反式作用和顺式作用区域
Cell. 1982 Oct;30(3):925-32. doi: 10.1016/0092-8674(82)90297-5.
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Processing of yeast mitochondrial RNA: involvement of intramolecular hybrids in splicing of cob intron 4 RNA by mutation and reversion.酵母线粒体RNA的加工：通过突变和回复突变研究分子内杂交体在细胞色素b氧化酶亚基1内含子4 RNA剪接中的作用
Cell. 1983 May;33(1):195-202. doi: 10.1016/0092-8674(83)90348-3.
10
Determination of functional domains in intron bI1 of yeast mitochondrial RNA by studies of mitochondrial mutations and a nuclear suppressor.通过线粒体突变和核抑制因子研究确定酵母线粒体RNA内含子bI1中的功能结构域
EMBO J. 1983;2(11):2047-52. doi: 10.1002/j.1460-2075.1983.tb01698.x.