Kim Miran, Kim Kwang Young, Nam Seung Won, Shin Woongghi, Yih Wonho, Park Myung Gil
LOHABE, Department of Oceanography, Chonnam National University, Gwangju, 500-757, Korea.
J Eukaryot Microbiol. 2014 Jul-Aug;61(4):354-63. doi: 10.1111/jeu.12115. Epub 2014 May 23.
The dinoflagellate Amylax triacantha is known to retain plastids of cryptophyte origin by engulfing the mixotrophic ciliate Mesodinium rubrum, itself a consumer of cryptophytes. However, there is no information on the fate of the prey's organelles and the photosynthetic performance of the newly retained plastids in A. triacantha. In this study, we conducted a starvation experiment to observe the intracellular organization of the prey's organelles and temporal changes in the photosynthetic efficiency of acquired plastids in A. triacantha. The ultrastructural observations revealed that while the chloroplast-mitochondria complexes and nucleus of cryptophyte were retained by A. triacantha, other ciliate organelles were digested in food vacuoles. Acquired plastids were retained in A. triacantha for about 1 mo and showed photosynthetic activities for about 18 d when measured by a pulse-amplitude modulation fluorometer.
已知甲藻Amylax triacantha通过吞噬混合营养型纤毛虫红色中缢虫（Mesodinium rubrum）来保留隐藻来源的质体，而红色中缢虫本身也是隐藻的消费者。然而，关于猎物细胞器的命运以及A. triacantha中新保留质体的光合性能，尚无相关信息。在本研究中，我们进行了一项饥饿实验，以观察猎物细胞器的细胞内组织以及A. triacantha中获得的质体光合效率的时间变化。超微结构观察表明，虽然A. triacantha保留了隐藻的叶绿体-线粒体复合体和细胞核，但其他纤毛虫细胞器在食物泡中被消化。通过脉冲幅度调制荧光计测量，获得的质体在A. triacantha中保留了约1个月，并显示出约18天的光合活性。
