Junera H R, Alfonsi M F, Fain-Maurel M A, Dadoune J P
Groupe d'Etude de la Formation et de la Maturation du Gamète Mâle, Laboratoires de Biologie cellulaire et d'Histologie, Paris, France.
Reprod Nutr Dev (1980). 1988;28(5):1267-73. doi: 10.1051/rnd:19880807.
The proteins of epididymal tissues and fluids recovered from five regions of the human epididymis were separated by polyacrylamide gel electrophoresis under denaturing conditions. Among the 60 peptides identified, eight appeared to be expressed solely in the epididymal duct when compared to serum and testis proteins. Three of these (92, 47 and 24 Kd) showed a degree of regional specificity in fluids. The 92 Kd peptide was found in the caput and proximal corpus, the 47 Kd in the distal corpus and cauda and the 24 Kd in the caput of the epididymis. Three of the specific epididymal proteins (39, 30, 26 Kd) displayed a remarkable analogy to those found in man and monkey in other conditions and which are present at the sperm surface in the epididymis cauda.
在变性条件下，通过聚丙烯酰胺凝胶电泳对从人类附睾五个区域回收的附睾组织和液体中的蛋白质进行分离。在鉴定出的60种肽中，与血清和睾丸蛋白质相比，有8种似乎仅在附睾管中表达。其中三种（92、47和24千道尔顿）在液体中表现出一定程度的区域特异性。92千道尔顿的肽存在于附睾头和附睾体近端，47千道尔顿的肽存在于附睾体远端和附睾尾，24千道尔顿的肽存在于附睾头。三种特异性附睾蛋白（39、30、26千道尔顿）与在其他情况下在人和猴子中发现的、存在于附睾尾精子表面的蛋白具有显著相似性。
