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聚丙烯酰胺凝胶电泳上人类附睾液蛋白质的模式

Patterns of human epididymal fluid proteins on polyacrylamide gel electrophoresis.

作者信息

Jennings M G, Temple-Smith P D, Southwick G J, Nauadu P L, Baker H W

出版信息

Int J Androl. 1987 Apr;10(2):441-6. doi: 10.1111/j.1365-2605.1987.tb00217.x.

DOI:10.1111/j.1365-2605.1987.tb00217.x
PMID:3610355
Abstract

Proteins were analysed by sodium dodecylsulphate polyacrylamide gel electrophoresis in fluid taken from six epididymal regions in 10 men undergoing microsurgery to bypass epididymal obstructions resulting from various disorders. Some major proteins common to most samples were identified with apparent molecular weights of 95, 67, 56, and 44 kilodaltons. A degree of regional specificity in the synthesis and secretion of epididymal proteins was indicated. There appeared to be no correlation between protein pattern or the abundance of individual proteins and the cause of obstruction, although methodological constraints may have partially obscured any such relationship.

摘要

对10名因各种疾病导致附睾梗阻而接受显微外科手术以绕过梗阻的男性,从六个附睾区域采集的液体中的蛋白质进行了十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析。在大多数样本中鉴定出一些主要蛋白质,其表观分子量分别为95、67、56和44千道尔顿。这表明附睾蛋白质的合成和分泌存在一定程度的区域特异性。尽管方法上的限制可能部分掩盖了蛋白质模式或单个蛋白质丰度与梗阻原因之间的任何关系,但两者之间似乎没有相关性。

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引用本文的文献

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Systematic mapping and functional analysis of a family of human epididymal secretory sperm-located proteins.系统绘制和功能分析人类附睾分泌精子定位蛋白家族。
Mol Cell Proteomics. 2010 Nov;9(11):2517-28. doi: 10.1074/mcp.M110.001719. Epub 2010 Aug 24.
2
Composition of fluids obtained from human epididymal cysts.从人附睾囊肿中获取的液体成分。
Urol Res. 1992;20(4):275-80. doi: 10.1007/BF00300258.