Eye Clinic, Department of Biomedical and Clinical Science, Luigi Sacco Hospital, University of Milan, Milan, Italy Università degli Studi di Milano, UO Oculistica, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milan, Italy.
Eye Clinic, Department of Biomedical and Clinical Science, Luigi Sacco Hospital, University of Milan, Milan, Italy.
Br J Ophthalmol. 2014 Sep;98(9):1245-9. doi: 10.1136/bjophthalmol-2013-304481. Epub 2014 Apr 15.
To assess the effectiveness of spectral domain optical coherence tomography (SD-OCT) in providing in vivo measurements of iris thickness in healthy and pathological subjects.
14 healthy volunteers and 14 patients with unilateral Fuchs' uveitis were enrolled in the study. The two groups were comparable for age, gender and race. Each subject underwent complete clinical examination and anterior segment SD-OCT imaging in both eyes. SD-OCT scans of the iris were performed following a cross-sectional pattern. Iris thickness values were obtained using a purposely developed software-based analysis of OCT images. Measurements were carried out twice by two trained independent operators to assess intraobserver and interobserver repeatability. Analysis of iris thickness was conducted in four main quadrants: superior, inferior, nasal and temporal. Iris thickness values from normal subjects were compared with the ones measured in the affected and fellow eyes of patients with Fuchs' uveitis.
Iris thickness measurements showed good intraobserver and interobserver repeatability (intraclass correlation coefficient >0.971). Superior and temporal iris sectors showed respectively thickest and thinnest values in all groups. In healthy eyes, iris thickness ranged from 327.92±37.29 μm temporally to 405.25±48.49 μm superiorly. Iris thickness measurements in the affected eyes of Fuchs' uveitis patients ranged from 285.48±56.02 μm temporally to 376.12±60.97 μm superiorly. Multiple comparison analysis showed iris thickness values to be significantly lower in eyes affected by Fuchs' uveitis than both in fellow eyes (p<0.001) of the same patients and in healthy eyes (p=0.0074).
SD-OCT is a suitable technique for iris thickness assessment. Thickness analysis must be carried out using a sectorial approach, taking into consideration anatomical variations existing between different iris regions. SD-OCT is a potentially useful tool for detecting iris thickness variations induced by pathological conditions such as Fuchs' uveitis.
评估频域光学相干断层扫描（SD-OCT）在活体测量健康和病理受试者虹膜厚度方面的有效性。
本研究纳入了 14 名健康志愿者和 14 名单侧 Fuchs 葡萄膜炎患者。两组在年龄、性别和种族方面具有可比性。所有受试者均接受了全面的临床检查和眼前节 SD-OCT 成像。SD-OCT 扫描采用横截面模式进行。使用专门开发的基于 OCT 图像的软件分析获得虹膜厚度值。由两名经过培训的独立操作人员进行两次测量，以评估观察者内和观察者间的可重复性。在四个主要象限（上、下、鼻和颞）进行虹膜厚度分析。将正常受试者的虹膜厚度值与 Fuchs 葡萄膜炎患者患眼和对侧眼的测量值进行比较。
虹膜厚度测量具有良好的观察者内和观察者间可重复性（组内相关系数>0.971）。上象限和颞象限的虹膜分别是所有组中最厚和最薄的部位。在健康眼中，虹膜厚度从颞侧的 327.92±37.29μm 到上侧的 405.25±48.49μm 不等。Fuchs 葡萄膜炎患者患眼的虹膜厚度测量值从颞侧的 285.48±56.02μm 到上侧的 376.12±60.97μm 不等。多重比较分析显示，Fuchs 葡萄膜炎患者患眼的虹膜厚度明显低于同一患者的对侧眼（p<0.001）和健康眼（p=0.0074）。
SD-OCT 是一种评估虹膜厚度的合适技术。必须采用扇形分析方法进行厚度分析，同时考虑到不同虹膜区域之间存在的解剖学差异。SD-OCT 是一种潜在有用的工具，可用于检测 Fuchs 葡萄膜炎等病理状态引起的虹膜厚度变化。
