Grizanova E V, Dubovskiy I M, Whitten M M A, Glupov V V
Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Science, Frunze Street 11, 630091 Novosibirsk, Russia.
Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Science, Frunze Street 11, 630091 Novosibirsk, Russia.
J Invertebr Pathol. 2014 Jun;119:40-6. doi: 10.1016/j.jip.2014.04.003. Epub 2014 Apr 13.
In this study the cellular and humoral immune reactions of the Greater wax moth Galleria mellonella have been investigated during bacterial infection caused by oral administration of Bacillus thuringiensis. Two different dose strengths were investigated to assess the contribution of immune parameters to induced Bt resistance. Low-dose (sublethal LC15) infection resulted in significantly elevated haemolymph phenoloxidase and lysozyme-like activity, enhanced phagocytic activity of haemocytes, and increased encapsulation responses in infected larvae at 48 and 72 h post infection. Higher doses of Bt (half-lethal LC50) also triggered significantly elevated haemolymph phenoloxidase and lysozyme-like activity, but decreased the coagulation index and activity of phenoloxidase in haemocytes of infected larvae. In both types of infection, the pool of circulating haemocytes became depleted. The importance of cellular and humoral immune reactions in induced insect resistance to intestinal bacterial infection Bt is herein discussed.
在本研究中，对大蜡螟（Galleria mellonella）经口服苏云金芽孢杆菌（Bacillus thuringiensis）引起细菌感染期间的细胞免疫和体液免疫反应进行了研究。研究了两种不同剂量强度，以评估免疫参数对诱导产生的苏云金芽孢杆菌抗性的贡献。低剂量（亚致死LC15）感染导致感染幼虫在感染后48小时和72小时时血淋巴酚氧化酶和类溶菌酶活性显著升高，血细胞吞噬活性增强，包囊反应增加。高剂量的苏云金芽孢杆菌（半致死LC50）也引发血淋巴酚氧化酶和类溶菌酶活性显著升高，但降低了感染幼虫血细胞中的凝血指数和酚氧化酶活性。在两种感染类型中，循环血细胞池均减少。本文讨论了细胞免疫和体液免疫反应在诱导昆虫对肠道细菌感染苏云金芽孢杆菌的抗性中的重要性。
