Patsch W, Brown S A, Morrisett J D, Gotto A M, Patsch J R
Baylor College of Medicine, Houston, TX 77030.
Clin Chem. 1989 Feb;35(2):265-70.
The dual-precipitation method for measurement of cholesterol in high-density lipoprotein subfractions HDL2 and HDL3 (Warnick et al., Clin Chem 1982;28:1574) was compared with quantification of cholesterol in HDL2 and HDL3 by zonal ultracentrifugation (Patsch et al., J Lipid Res 1974;15:356-66). For 39 plasma specimens differing widely in their HDL subfraction cholesterol concentration, the coefficient of correlation between the two methods was 0.94 for HDL2-cholesterol, 0.82 for HDL3-cholesterol. Storage of plasma specimens at -70 degrees C decreased the apparent content of HDL3-cholesterol by 5%; no significant changes in HDL2-cholesterol were observed. In frozen plasma, interference by apoB-containing lipoproteins and by lipoprotein(a) was negligible. Mean weight ratios of apoA-I to cholesterol were twice as high for HDL3 as for HDL2, reflecting the increased cholesterol content of HDL2. The study suggests that quantification of HDL2 and HDL3 cholesterol by precipitation is appropriate for use in epidemiological studies.
将用于测量高密度脂蛋白亚组分HDL2和HDL3中胆固醇的双沉淀法（Warnick等人，《临床化学》1982年；28:1574）与通过区带超速离心法对HDL2和HDL3中的胆固醇进行定量分析的方法（Patsch等人，《脂质研究杂志》1974年；15:356 - 66）进行了比较。对于39份HDL亚组分胆固醇浓度差异很大的血浆标本，两种方法之间的相关系数对于HDL2 - 胆固醇为0.94，对于HDL3 - 胆固醇为0.82。血浆标本在 - 70℃下储存使HDL3 - 胆固醇的表观含量降低了5%；未观察到HDL2 - 胆固醇有显著变化。在冷冻血浆中，含载脂蛋白B的脂蛋白和脂蛋白(a)的干扰可忽略不计。HDL3的载脂蛋白A - I与胆固醇的平均重量比是HDL2的两倍，这反映了HDL2中胆固醇含量的增加。该研究表明，通过沉淀法对HDL2和HDL3胆固醇进行定量分析适用于流行病学研究。
