Shukla D D, Ford R E, Tosic M, Jilka J, Ward C W
CSIRO, Division of Biotechnology, Parkville Laboratory, Victoria, Australia.
Arch Virol. 1989;105(3-4):143-51. doi: 10.1007/BF01311352.
There are at least ten viruses identified in the literature that resemble definitive potyviruses in having flexuous filamentous particles and inducing the formation of "pinwheel" cytoplasmic inclusions in infected cells but that are transmitted by eriophyid mites, whiteflies or soil fungi and not by aphids, the vectors of the definitive potyviruses. The taxonomic status of these viruses is uncertain at present. Using a broadly cross-reactive antiserum raised against the dissociated coat protein core (residues 68-285) of a definitive potyvirus (Johnsongrass mosaic virus), we have shown that wheat streak mosaic virus which is transmitted by mite and sweet potato mild mottle virus which is transmitted by whitefly have coat proteins that share epitopes with definitive potyviruses. This finding further supports their classification as definitive members of the potyvirus group. The cross-reactive antiserum used here had been shown previously to react with coat proteins of fifteen different definitive potyviruses. The antiserum did not react with coat proteins of potexviruses and tobamoviruses.
文献中已鉴定出至少十种病毒，它们具有弯曲的丝状颗粒，在感染细胞中诱导形成“风车状”细胞质内含物，类似于典型的马铃薯Y病毒，但由瘿螨、粉虱或土壤真菌传播，而非典型马铃薯Y病毒的传播媒介蚜虫传播。目前这些病毒的分类地位尚不确定。利用针对一种典型马铃薯Y病毒（高粱花叶病毒）解离的衣壳蛋白核心（第68 - 285位氨基酸残基）产生的广泛交叉反应抗血清，我们发现由螨传播的小麦条纹花叶病毒和由粉虱传播的甘薯轻度斑驳病毒的衣壳蛋白与典型马铃薯Y病毒具有共同表位。这一发现进一步支持将它们归类为马铃薯Y病毒组的典型成员。此处使用的交叉反应抗血清先前已显示能与十五种不同典型马铃薯Y病毒的衣壳蛋白发生反应。该抗血清与马铃薯X病毒属和烟草花叶病毒属的衣壳蛋白不发生反应。
