O'Donnell I J, Shukla D D, Gough K H
J Virol Methods. 1982 Feb;4(1):19-26. doi: 10.1016/0166-0934(82)90050-7.
A new technique for detecting viruses in plant sap is described. It consists of sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the infected plant sap, electrophoretic transfer of protein bands to activated paper by the Electro-Blot technique, the subsequent probing of the viral coat protein band by specific antiserum (prepared against intact virus), and detection of immune complex with 125 I-labelled protein A. The technique successfully detected tobacco mosaic virus at a sap dilution of 1 : 10,000, four strains of sugarcane mosaic virus (a potyvirus) in their perennial hosts infected for about 4 years, and five different isolated of potato leaf roll virus (a luteovirus). The latter virus occurs in extremely low concentration and is difficult to detect by the other known methods.
本文描述了一种检测植物汁液中病毒的新技术。该技术包括对受感染植物汁液进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，通过电印迹技术将蛋白带电泳转移至活化纸上，随后用特异性抗血清（针对完整病毒制备）探测病毒衣壳蛋白带，并用¹²⁵I标记的蛋白A检测免疫复合物。该技术成功检测到了稀释度为1:10000的烟草花叶病毒、在多年生寄主中感染约4年的4种甘蔗花叶病毒株系（一种马铃薯Y病毒属病毒）以及5种不同分离株的马铃薯卷叶病毒（一种黄症病毒属病毒）。后一种病毒浓度极低，用其他已知方法难以检测。
