Domier L L, Franklin K M, Shahabuddin M, Hellmann G M, Overmeyer J H, Hiremath S T, Siaw M F, Lomonossoff G P, Shaw J G, Rhoads R E
Nucleic Acids Res. 1986 Jul 11;14(13):5417-30. doi: 10.1093/nar/14.13.5417.
The nucleotide sequence of the RNA of tobacco vein mottling virus, a member of the potyvirus group, was determined. The RNA was found to be 9471 residues in length, excluding a 3'-terminal poly(A) tail. The first three AUG codons from the 5'-terminus were followed by in-frame termination codons. The fourth, at position 206, was the beginning of an open reading frame of 9015 residues which could encode a polyprotein of 340 kDa. No other long open reading frames were present in the sequence or its complement. This AUG was present in the sequence AGGCCAUG, which is similar to the consensus initiation sequence shared by most eukaryotic mRNAs. The chemically-determined amino acid compositions of the helper component and coat proteins were similar to those predicted from the nucleotide sequence. Amino acid sequencing of coat protein from which an amino-terminal peptide had been removed allowed exact location of the coat protein cistron. A consensus sequence of V-(R or K)-F-Q was found on the N-terminal sides of proposed cleavage sites for proteolytic processing of the polyprotein.
烟草脉斑驳病毒（马铃薯Y病毒组的一个成员）的RNA核苷酸序列已被测定。发现该RNA长度为9471个残基，不包括3'末端的聚（A）尾。从5'末端起的前三个AUG密码子之后是符合读框的终止密码子。第四个位于206位，是一个9015个残基的开放阅读框的起始，该开放阅读框可编码一个340 kDa的多聚蛋白。在该序列或其互补序列中不存在其他长开放阅读框。这个AUG存在于序列AGGCCAUG中，它与大多数真核mRNA共有的共有起始序列相似。辅助成分和外壳蛋白的化学测定氨基酸组成与从核苷酸序列预测的相似。对去除了氨基末端肽的外壳蛋白进行氨基酸测序，确定了外壳蛋白顺反子的确切位置。在多聚蛋白进行蛋白水解加工的假定切割位点的N末端一侧发现了一个V-（R或K）-F-Q共有序列。
