源自日本扇贝（虾夷扇贝）表达序列标签的微卫星标记。

Microsatellite markers derived from Japanese scallop (Mizuhopecten yessoensis) expressed sequence tags.

作者信息

Li Y F, Gao X G, Liu W D, Tian Y, Gao L, Li S L, He C B

机构信息

Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.

College of Fisheries and Life Science, Dalian Ocean University, Dalian, China.

出版信息

Genet Mol Res. 2014 Mar 24;13(1):1989-92. doi: 10.4238/2014.March.24.3.

DOI:10.4238/2014.March.24.3

PMID:24737424

Abstract

Japanese scallop (Mizuhopecten yessoensis) is a cold-water shellfish, and a species of economic importance in China. In this study, we developed and evaluated simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs) of M. yessoensis. The characteristics of 12 EST-SSR loci were investigated in 30 individual scallops, and the result revealed that the number of alleles per locus ranged from 2-4, with an observed heterozygosity ranging from 0.0333-0.7692, and an expected heterozygosity ranging from 0.0333-0.6312. Only two loci were found to depart significantly from the Hardy-Weinberg equilibrium (P < 0.05). The result of our study suggested that these markers could be considered as potential markers for studying the population structure of M. yessoensis and its intraspecific variation.

摘要

日本扇贝（虾夷扇贝）是一种冷水贝类，在中国具有重要经济价值。在本研究中，我们从虾夷扇贝的表达序列标签（EST）中开发并评估了简单序列重复（SSR）标记。在30个个体扇贝中研究了12个EST-SSR位点的特征，结果显示每个位点的等位基因数为2-4个，观察到的杂合度范围为0.0333-0.7692，预期杂合度范围为0.0333-0.6312。仅发现两个位点显著偏离哈迪-温伯格平衡（P < 0.05）。我们的研究结果表明，这些标记可被视为研究虾夷扇贝种群结构及其种内变异的潜在标记。