Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
Am J Bot. 2012 Oct;99(10):e379-90. doi: 10.3732/ajb.1100346. Epub 2012 Oct 1.
Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. •
Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. •
These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea.
通过筛选美国国家生物技术信息中心（NCBI）数据库，从野豌豆中开发出基于表达序列标签（EST）的微卫星标记。这些新标记的有效性已通过对来自世界各地的24 个野豌豆品种的大小多态性进行验证。
从全球范围内的 24 个野豌豆品种中，鉴定了 300 对基于表达序列标签的简单重复序列（SSR）引物对，并对其大小多态性进行了特征描述。其中，139 个 SSR 位点没有产生 PCR 产物，117 个 SSR 位点是单态的，44 个 SSR 位点是多态的。每个位点的等位基因数平均从两个到十一个不等。观察到的杂合度和期望杂合度分别在 0.000 到 1.000 和 0.042 到 0.836 之间。
这些新的标记将有助于野豌豆的遗传图谱和分子育种研究。
