Matsumine Hajime, Sasaki Ryo, Tabata Yasuhiko, Matsui Makoto, Yamato Masayuki, Okano Teruo, Sakurai Hiroyuki
Department of Plastic Surgery, and Global Center of Excellence (COE) Program, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
J Tissue Eng Regen Med. 2016 Oct;10(10):E559-E567. doi: 10.1002/term.1884. Epub 2014 Apr 16.
Basic fibroblast growth factor (bFGF) plays a crucial role in the regeneration of peripheral nerve defects by affecting nerve cells, Schwann cells and fibroblasts, and by promoting axon outgrowth from the proximal nerve stump. However, the use of exogenous bFGF for in vivo regeneration of the peripheral nerves is limited by its short in vivo half-life. In this study, a drug delivery system for bFGF was developed that uses acidic gelatin hydrogel, which sustainably released bFGF in vivo over several weeks; its ability to promote peripheral nerve regeneration was also examined. In 8-week-old Lewis rats, 7-mm gaps were made in the buccal branch of the left facial nerve. Acidic gelatin hydrogel microspheres (10 µl) with or without bFGF (50 µg) were infused into a 10 mm silicone tube using a micropipette, and the silicone tube was then implanted into the gap. A 1-mm long nerve stump was inserted into each end of the tube. Histological examination at 7 weeks after implantation revealed (1) a significantly increased rate of nerve regeneration, (2) inducement of a number of regenerating nerve axons, and (3) a better degree of maturation of nerve axons in the bFGF microsphere group than that in the bFGF-free microsphere group. Copyright © 2014 John Wiley & Sons, Ltd.
碱性成纤维细胞生长因子（bFGF）通过影响神经细胞、雪旺细胞和成纤维细胞，并促进轴突从近端神经残端长出，在周围神经缺损的再生中发挥关键作用。然而，外源性bFGF用于周围神经的体内再生受到其体内半衰期短的限制。在本研究中，开发了一种用于bFGF的药物递送系统，该系统使用酸性明胶水凝胶，其可在体内数周内持续释放bFGF；还检测了其促进周围神经再生的能力。在8周龄的Lewis大鼠中，在左侧面神经颊支制造7毫米的间隙。使用微量移液器将含有或不含有bFGF（50微克）的酸性明胶水凝胶微球（10微升）注入10毫米的硅胶管中，然后将硅胶管植入间隙。将1毫米长的神经残端插入管的两端。植入后7周的组织学检查显示：（1）神经再生率显著提高；（2）诱导了许多再生神经轴突；（3）bFGF微球组神经轴突的成熟程度优于无bFGF微球组。版权所有©2014约翰威立父子有限公司。
