Duan R D, Poensgen J, Wicker C, Weström B, Erlanson-Albertsson C
Department of Medical and Physiological Chemistry, University of Lund, Sweden.
Dig Dis Sci. 1989 Aug;34(8):1243-8. doi: 10.1007/BF01537273.
The pancreatic lipase and trypsin activities in streptozotocin-induced diabetic rats were determined as well as the relative levels of mRNA coding for these proteins. It was found that after development of diabetes, the activities of pancreatic lipase and trypsin were significantly increased by 105% and 52%, respectively, accompanied by an increase in the levels of lipase and trypsinogen mRNA by 98% and 49%, respectively, while amylase activity and its mRNA were significantly decreased. The alteration of lipase, trypsin, and amylase activities and their mRNA in diabetic rats were all normalized by replacement of insulin. It is concluded that in the diabetic situation, the pretranslational control for pancreatic lipase and trypsinogen is stimulated, resulting in high levels of these enzymes in the diabetic rat.
测定了链脲佐菌素诱导的糖尿病大鼠的胰腺脂肪酶和胰蛋白酶活性以及编码这些蛋白质的mRNA的相对水平。结果发现，糖尿病发生后，胰腺脂肪酶和胰蛋白酶的活性分别显著增加了105%和52%，同时脂肪酶和胰蛋白酶原mRNA水平分别增加了98%和49%，而淀粉酶活性及其mRNA则显著降低。糖尿病大鼠脂肪酶、胰蛋白酶和淀粉酶活性及其mRNA的改变通过胰岛素替代均恢复正常。得出结论，在糖尿病状态下，胰腺脂肪酶和胰蛋白酶原的翻译前控制受到刺激，导致糖尿病大鼠中这些酶的水平升高。
