博来霉素耐药的HeLa细胞中多聚（ADP-核糖）聚合酶活性的高诱导。
High induction of poly(ADP-ribose) polymerase activity in bleomycin-resistant HeLa cells.
作者信息
Urade M, Sugi M, Mima T, Ogura T, Matsuya T
机构信息
First Department of Oral and Maxillofacial Surgery, Osaka University Faculty of Dentistry.
出版信息
Jpn J Cancer Res. 1989 May;80(5):464-8. doi: 10.1111/j.1349-7006.1989.tb02337.x.
Poly(ADP-ribose) polymerase activity was measured in bleomycin (BLM)-resistant HeLa (HeLa-BLMr) and the parental HeLa cells after BLM treatment. HeLa-BLMr cells, which had been subcultured in growth medium containing 1 micrograms/ml of BLM, showed a 3.75-fold higher enzyme activity than did HeLa cells, but this activity was decreased to the same level as that of HeLa cells after 48 h of BLM-free cultivation. When HeLa and HeLa-BLMr cells after a 48-h cultivation in BLM-free growth medium were treated with BLM, the enzyme activity was induced at a higher level (2.5-3.8 times) in HeLa-BLMr than in HeLa cells and was inhibited markedly in HeLa-BLMr and slightly in HeLa cells by nicotinamide, an inhibitor of this enzyme. The BLM-induced cell killing by nicotinamide was highly potentiated (about 18 times) in HeLa-BLMr as compared to HeLa cells.
在博来霉素（BLM）处理后，测定了耐博来霉素的HeLa细胞（HeLa - BLMr）及其亲代HeLa细胞中的聚（ADP - 核糖）聚合酶活性。在含有1微克/毫升博来霉素的生长培养基中传代培养的HeLa - BLMr细胞，其酶活性比HeLa细胞高3.75倍，但在无博来霉素培养48小时后，该活性降至与HeLa细胞相同的水平。当在无博来霉素的生长培养基中培养48小时后的HeLa细胞和HeLa - BLMr细胞用博来霉素处理时，HeLa - BLMr细胞中的酶活性诱导水平比HeLa细胞更高（2.5 - 3.8倍），并且该酶的抑制剂烟酰胺可显著抑制HeLa - BLMr细胞中的酶活性，对HeLa细胞中的酶活性抑制作用较弱。与HeLa细胞相比，烟酰胺对博来霉素诱导的HeLa - BLMr细胞杀伤作用有高度增强（约18倍）。
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