Chen Xiao-Ping, Chen Wei-Feng, Wang Da-Wei
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, China.
PLoS One. 2014 Apr 16;9(4):e95343. doi: 10.1371/journal.pone.0095343. eCollection 2014.
Prenatal organophosphate exposure elicits long-term brain cytoarchitecture and cognitive function impairments, but the mechanism underlying the onset and development of neural progenitors remain largely unclear. Using precise positioned brain slices, we observed an alternated cleavage plane bias that emerged in the mitotic neural progenitors of embryonal neocortex with diazinion (DZN) and chlorpyrifos (CPF) pretreatment. In comparison with the control, DZN and CPF treatment induced decrease of vertical orientation, increase of oblique orientation, and increase of horizontal orientation. That is, the cleavage plane orientation bias had been rotated from vertical to horizontal after DZN and CPF treatment. Meanwhile, general morphology and mitotic index of the progenitors were unchanged. Acephate (ACP), another common organophosphate, had no significant effects on the cleavage plane orientation, cell morphology and mitotic index. These results represent direct evidence for the toxicity mechanism in onset multiplication of neural progenitors.
产前有机磷暴露会引发长期的脑细胞结构和认知功能损害，但神经祖细胞发病和发展的潜在机制仍不清楚。我们使用精确定位的脑切片，观察到在使用二嗪农（DZN）和毒死蜱（CPF）预处理的胚胎新皮质有丝分裂神经祖细胞中出现了交替的分裂平面偏向。与对照组相比，DZN和CPF处理导致垂直取向减少、倾斜取向增加和水平取向增加。也就是说，在DZN和CPF处理后，分裂平面取向偏向已从垂直旋转到水平。同时，祖细胞的一般形态和有丝分裂指数没有变化。另一种常见的有机磷乙酰甲胺磷（ACP）对分裂平面取向、细胞形态和有丝分裂指数没有显著影响。这些结果为神经祖细胞起始增殖的毒性机制提供了直接证据。
