Gahlout Mayur, Gupte Shilpa, Gupte Akshaya
Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, Gujarat, 388 120, India.
Appl Biochem Biotechnol. 2014 May;173(1):215-27. doi: 10.1007/s12010-014-0835-0. Epub 2014 Apr 17.
In the present study, three different types of hydrogels i.e., (poly (-acrylamide)/alginate (P (AAm)/Alg), poly (acrylamide-N-isopropylacrylamide) (P (AAm-NIPA)), and poly (acrylamide-N-isopropylacrylamide)/alginate (P (AAm-NIPA)/Alg)) were synthesized by acrylamide, alginate, and N-isopropylacrylamide for the entrapment of laccase. The hydrogel-entrapped and free laccase showed optimum temperature of 50 °C for the oxidation of ABTS, but the entrapped laccase showed high temperature, pH, and storage stability as compared to the free enzyme. The K m values of free laccase, (P (AAm)/Alg)-L, (P (AAm-NIPA))-L, and (P (AAm-NIPA)/Alg)-L were found to be 0.13, 0.28, 0.33, and 0.50 mM, respectively. The V max values of free laccase, (P (AAm)/Alg)-L, (P (AAm-NIPA))-L, and (P (AAm-NIPA)/Alg)-L were found to be 22.22 × 10(2), 5.55 × 10(2), 5.0 × 10(2), and 4.54 × 10(2) mM/min, respectively. The entrapped laccase hydrogels were used for the decolorization of Reactive Violet 1 dye, with 39 to 45 % decolorization efficiency till the 10th cycle.
在本研究中，通过丙烯酰胺、海藻酸盐和N-异丙基丙烯酰胺合成了三种不同类型的水凝胶，即聚（丙烯酰胺）/海藻酸盐（P（AAm）/Alg）、聚（丙烯酰胺-N-异丙基丙烯酰胺）（P（AAm-NIPA））和聚（丙烯酰胺-N-异丙基丙烯酰胺）/海藻酸盐（P（AAm-NIPA）/Alg），用于包埋漆酶。包埋在水凝胶中的漆酶和游离漆酶对ABTS氧化的最适温度均为50℃，但与游离酶相比，包埋的漆酶表现出更高的温度稳定性、pH稳定性和储存稳定性。游离漆酶、（P（AAm）/Alg）-L、（P（AAm-NIPA））-L和（P（AAm-NIPA）/Alg）-L的米氏常数（K m）分别为0.13、0.28、0.33和0.50 mM。游离漆酶、（P（AAm）/Alg）-L、（P（AAm-NIPA））-L和（P（AAm-NIPA）/Alg）-L的最大反应速度（V max）分别为22.22×10²、5.55×10²、5.0×10²和4.54×10² mM/min。包埋漆酶的水凝胶用于活性紫1染料的脱色，直到第10个循环，脱色效率为39%至45%。
