髓鞘碱性蛋白的T细胞决定簇包括一个针对Lewis大鼠的独特的受I-E限制的致脑炎表位。
T cell determinants of myelin basic protein include a unique encephalitogenic I-E-restricted epitope for Lewis rats.
作者信息
Offner H, Hashim G A, Celnik B, Galang A, Li X B, Burns F R, Shen N, Heber-Katz E, Vandenbark A A
机构信息
Veterans Administration Medical Center, Portland, Oregon 97201.
出版信息
J Exp Med. 1989 Aug 1;170(2):355-67. doi: 10.1084/jem.170.2.355.
The major encephalitogenic epitope for Lewis rats is the 72-89 sequence of guinea pig basic protein (GP-BP) or rat basic protein (Rt-BP). T cells responsive to this epitope are I-A restricted and preferentially express the V alpha 2:V beta 8 gene combination in their TCR. In this work, we describe for the first time the delayed appearance of T cells specific for additional discrete determinant of BP, the nonencephalitogenic 55-68 sequence of GP-BP restricted by I-A, and the encephalitogenic 87-99 sequence of Rt-BP restricted by I-E. The TCR V alpha 2:V beta 8 gene combination was expressed by both encephalitogenic GP-BP S72-89 and Rt-BP S87-99 T cell specificities but not by GP-BP 44-68-specific T cells. This is the first demonstration of I-E-restricted encephalitogenic T cells in Lewis rats and supports the conclusion that the I-E class II locus is involved in autoimmune diseases.
对Lewis大鼠而言，主要的致脑炎性表位是豚鼠碱性蛋白（GP-BP）或大鼠碱性蛋白（Rt-BP）的72-89序列。对该表位有反应的T细胞受I-A限制，并且在其TCR中优先表达Vα2:Vβ8基因组合。在本研究中，我们首次描述了对BP其他离散决定簇具有特异性的T细胞的延迟出现，即受I-A限制的GP-BP的非致脑炎性55-68序列，以及受I-E限制的Rt-BP的致脑炎性87-99序列。致脑炎性的GP-BP S72-89和Rt-BP S87-99 T细胞特异性均表达TCR Vα2:Vβ8基因组合，而GP-BP 44-68特异性T细胞则不表达。这是首次在Lewis大鼠中证实受I-E限制的致脑炎性T细胞，并支持II类I-E基因座参与自身免疫性疾病的结论。
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