Olstad Kristin, Kongsro Jørgen, Grindflek Eli, Dolvik Nils Ivar
Norwegian School of Veterinary Science, Companion Animal Clinical Sciences, Equine Section, Ullevålsveien 72, 0454 Oslo, Norway.
J Orthop Res. 2014 Aug;32(8):1014-23. doi: 10.1002/jor.22631. Epub 2014 Apr 17.
The purpose of the current study was to validate the use of CT for selection against osteochondrosis in pigs by calculating positive predictive value and comparing it to the positive predictive value of macroscopic evaluation, using histological examination as the reference standard. Eighteen male, hereditarily osteochondrosis-predisposed piglets underwent terminal examination at biweekly intervals from the ages of 82-180 days old, including CT scanning, macroscopic, and histological evaluation of the left distal femur. Areas of ischemic chondronecrosis (osteochondrosis) were confirmed in histological sections from 44/56 macroscopically suspected lesions, resulting in a positive predictive value of 79% (95% CI: 67-84%). Suspected lesions, that is; focal, radiolucent defects in the ossification front in CT scans corresponded to areas of ischemic chondronecrosis in 36/36 histologically examined lesions, resulting in a positive predictive value of 100% (95% CI: 90-100%). CT was superior to macroscopic evaluation for diagnosis of early stages of osteochondrosis in the distal femur of piglets. The current histologically validated observations can potentially be extrapolated to diagnostic monitoring of juvenile osteochondritis dissecans in children, or to animal models of human juvenile articular cartilage injury and repair.
本研究的目的是通过计算阳性预测值，并将其与宏观评估的阳性预测值进行比较，以组织学检查作为参考标准，验证CT在筛选易患骨软骨病的猪方面的应用。18只遗传性易患骨软骨病的雄性仔猪在82至180日龄期间每两周接受一次终末检查，包括对左股骨远端进行CT扫描、宏观评估和组织学评估。在44/56个宏观上疑似病变的组织学切片中证实了缺血性软骨坏死（骨软骨病）区域，阳性预测值为79%（95%CI：67-84%）。疑似病变，即在CT扫描中骨化前沿的局灶性、射线可透性缺损，在36/36个经组织学检查的病变中对应于缺血性软骨坏死区域，阳性预测值为100%（95%CI：90-100%）。在诊断仔猪股骨远端骨软骨病的早期阶段，CT优于宏观评估。目前经组织学验证的观察结果可能会外推至儿童青少年剥脱性骨软骨炎的诊断监测，或人类青少年关节软骨损伤与修复的动物模型。
