Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, People's Republic of China ; Graduate School of Dentistry (Removable Prosthodontics and Occlusion), Osaka Dental University, Hirakata, Osaka, Japan.
Department of Removable Prosthodontics and Occlusion, Osaka Dental University, Hirakata, Osaka, Japan.
Int J Nanomedicine. 2014 Apr 5;9:1741-55. doi: 10.2147/IJN.S58502. eCollection 2014.
Titanium surfaces play an important role in affecting osseointegration of dental implants. Previous studies have shown that the titania nanotube promotes osseointegration by enhancing osteogenic differentiation. Only relatively recently have the effects of titanium surfaces with other nanostructures on osteogenic differentiation been investigated.
In this study, we used NaOH solutions with concentrations of 2.5, 5.0, 7.5, 10.0, and 12.5 M to develop a simple and useful titanium surface modification that introduces the nanonetwork structures with titania nanosheet (TNS) nanofeatures to the surface of titanium disks. The effects of such a modified nanonetwork structure, with different alkaline concentrations on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMMSCs), were evaluated.
The nanonetwork structures with TNS nanofeatures induced by alkali etching markedly enhanced BMMSC functions of cell adhesion and osteogenesis-related gene expression, and other cell behaviors such as proliferation, alkaline phosphatase activity, extracellular matrix deposition, and mineralization were also significantly increased. These effects were most pronounced when the concentration of NaOH was 10.0 M.
The results suggest that nanonetwork structures with TNS nanofeatures improved BMMSC proliferation and induced BMMSC osteogenic differentiation. In addition, the surfaces formed with 10.0 M NaOH suggest the potential to improve the clinical performance of dental implants.
钛表面在影响牙种植体骨整合方面起着重要作用。以前的研究表明，二氧化钛纳米管通过增强成骨分化来促进骨整合。直到最近，人们才研究了具有其他纳米结构的钛表面对成骨分化的影响。
在这项研究中，我们使用浓度为 2.5、5.0、7.5、10.0 和 12.5 M 的 NaOH 溶液在钛盘表面开发了一种简单而有用的钛表面改性方法，引入具有二氧化钛纳米片（TNS）纳米结构的纳米网络结构。评估了具有不同碱浓度的这种改性纳米网络结构对大鼠骨髓间充质干细胞（BMMSCs）成骨分化的影响。
碱刻蚀诱导的纳米网络结构具有 TNS 纳米结构，明显增强了 BMMSC 的细胞黏附功能和成骨相关基因表达，其他细胞行为，如增殖、碱性磷酸酶活性、细胞外基质沉积和矿化也显著增加。当 NaOH 浓度为 10.0 M 时，效果最为明显。
结果表明，具有 TNS 纳米结构的纳米网络结构改善了 BMMSC 的增殖，并诱导了 BMMSC 的成骨分化。此外，用 10.0 M NaOH 形成的表面表明有可能改善牙科植入物的临床性能。
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