Matsumoto Takumi, Tashiro Yuichiro, Komasa Satoshi, Miyake Akiko, Komasa Yutaka, Okazaki Joji
Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan.
Department of Japan Faculty of Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 573-1121, Japan.
Materials (Basel). 2020 Dec 28;14(1):97. doi: 10.3390/ma14010097.
Primary stability and osseointegration are major challenges in dental implant treatments, where the material surface properties and wettability are critical in the early formation of hard tissue around the implant. In this study, a quartz crystal microbalance (QCM) was used to measure the nanogram level amount of protein and bone marrow cells adhered to the surfaces of titanium (Ti) surface in real time. The effects of ultraviolet (UV) and atmospheric-pressure plasma treatment to impart surface hydrophilicity to the implant surface were evaluated. The surface treatment methods resulted in a marked decrease in the surface carbon (C) content and increase in the oxygen (O) content, along with super hydrophilicity. The results of QCM measurements showed that adhesion of both adhesive proteins and bone marrow cells was enhanced after surface treatment. Although both methods produced implants with good osseointegration behavior and less reactive oxidative species, the samples treated with atmospheric pressure plasma showed the best overall performance and are recommended for clinical use. It was verified that QCM is an effective method for analyzing the initial adhesion process on dental implants.
初期稳定性和骨结合是牙种植治疗中的主要挑战，其中材料表面特性和润湿性对于种植体周围硬组织的早期形成至关重要。在本研究中，使用石英晶体微天平（QCM）实时测量附着在钛（Ti）表面的纳克级蛋白质和骨髓细胞数量。评估了紫外线（UV）和大气压等离子体处理赋予种植体表面亲水性的效果。表面处理方法导致表面碳（C）含量显著降低，氧（O）含量增加，同时具有超亲水性。QCM测量结果表明，表面处理后黏附蛋白和骨髓细胞的黏附均增强。尽管两种方法都产生了具有良好骨结合行为且活性氧化物种较少的种植体，但大气压等离子体处理的样品总体性能最佳，推荐用于临床。证实了QCM是分析牙种植体初始黏附过程的有效方法。
