Zhang Liang, Fu Chong-Luo
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2014 Jan;30(1):33-6.
Established with amphotericin B perforated patch-clamp technique, to study the electrophysiological properties of calyx synapses.
In the present experiments, we studied the application of perforated patch clamp technique on the calyx synapses of mice with Amphotericin B.
The use of Amphotericin B significantly slowed down the decay of channel currents and the optimum concentration was 400 microg/ml.
The syudy developed a stable of perforated patch clamp whole cell recording technique, could be more effecitve, more real reaction neurons electrophysiological characteristics of the channel current. Our work might provide the basic information to future users studying the signal transmission and regulation of auditory system of rodents.
采用两性霉素B穿孔膜片钳技术，研究花萼突触的电生理特性。
在本实验中，我们研究了两性霉素B穿孔膜片钳技术在小鼠花萼突触上的应用。
两性霉素B的使用显著减缓了通道电流的衰减，最佳浓度为400微克/毫升。
本研究建立了稳定的穿孔膜片钳全细胞记录技术，能更有效、更真实地反映神经元通道电流的电生理特性。我们的工作可能为未来研究啮齿动物听觉系统信号传递和调节的人员提供基础信息。
