一种用于筛选链霉菌产生的胞外柚皮苷酶的简单平板测定法。
A simple plate-assay for screening extracellular naringinase produced by streptomycetes.
作者信息
Caraveo Leonela, Medina Héctor, Rodríguez-Buenfil Ingrid, Montalvo-Romero Carlos, Evangelista-Martínez Zahaed
机构信息
Universidad Autónoma del Carmen, Ciudad del Carmen, Campeche México.
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ, A.C.) Unidad Sureste, Yucatán, México.
出版信息
J Microbiol Methods. 2014 Jul;102:8-11. doi: 10.1016/j.mimet.2014.04.003. Epub 2014 Apr 15.
A simple plate-assay was developed with the purpose of detecting extracellular naringinase in streptomycetes. The naringin agar medium (NAM) was prepared by mixing carbon utilization medium (ISP9) and naringin. The clearing zones around colonies are correlated with the enzymatic activity. The assay validation was performed through the use of commercial naringinase (Penicillium decumbens).
为检测链霉菌中的胞外柚皮苷酶，开发了一种简单的平板测定法。柚皮苷琼脂培养基（NAM）通过将碳源利用培养基（ISP9）和柚皮苷混合制备而成。菌落周围的透明圈与酶活性相关。通过使用商业柚皮苷酶（斜卧青霉）进行测定法验证。
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