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一种筛选潜在产柚苷酶微生物的新方法。

A novel screening method for potential naringinase-producing microorganisms.

作者信息

Patil Satish V, Koli Sunil H, Mohite Bhavana V, Patil Rahul P, Patil Rohini R, Borase Hemant P, Patil Vikas S

机构信息

School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India.

North Maharashtra Microbial Culture Collection Centre (NMCC), Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India.

出版信息

Biotechnol Appl Biochem. 2019 May;66(3):323-327. doi: 10.1002/bab.1728. Epub 2019 Jan 22.

Abstract

Naringinase has high industrial importance, and the progress in naringinase research is still quite slow. The unavailability of an effective, simple screening method, which will be applicable to different microorganisms such as bacteria, fungi, and actinomycetes, is one of the main reasons for this gap. Therefore, a simple plate assay was developed for effective screening of microorganisms for naringinase by exposing to iodine vapors. This plate assay will fill the technological void for simple screening method and will lead to screen more potent industrially important naringinase-producing microorganisms.

摘要

柚苷酶具有很高的工业重要性,然而柚苷酶的研究进展仍然相当缓慢。缺乏一种适用于不同微生物(如细菌、真菌和放线菌)的有效、简单的筛选方法是造成这一差距的主要原因之一。因此,开发了一种简单的平板测定法,通过暴露于碘蒸气来有效筛选产柚苷酶的微生物。这种平板测定法将填补简单筛选方法的技术空白,并有助于筛选出更具潜力的具有工业重要性的产柚苷酶微生物。

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