NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok 690022, Russia.
Int J Syst Evol Microbiol. 2014 Jul;64(Pt 7):2370-2375. doi: 10.1099/ijs.0.061747-0. Epub 2014 Apr 17.
An aerobic, Gram-stain-negative, non-motile bacterium, KMM 9530(T), was isolated from a sediment sample collected from the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis positioned novel strain KMM 9530(T) in the genus Loktanella as a separate line adjacent to Loktanella sediminilitoris KCTC 32383(T), Loktanella tamlensis JCM 14020(T) and Loktanella maricola JCM 14564(T) with 98.5-98.2% sequence similarity. Strain KMM 9530(T) was characterized by its weak hydrolytic capacity and inability to assimilate most organic substrates. The major isoprenoid quinone was Q-10, polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid, an unknown aminolipid and unknown lipids, and the major fatty acid was C18 : 1ω7c. On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotypic characterization, it can be concluded that the novel strain KMM 9530(T) represents a novel species in the genus Loktanella, for which the name Loktanella maritima sp. nov. is proposed. The type strain of the species is KMM 9530(T) ( = NRIC 0919(T) = JCM 19807(T)).
一株好氧、革兰氏阴性、非运动的细菌，KMM 9530(T)，从日本海海岸的沉积物样本中分离得到。比较 16S rRNA 基因序列分析将新菌株 KMM 9530(T)置于 Loktanella 属中，与 Loktanella sediminilitoris KCTC 32383(T)、Loktanella tamlensis JCM 14020(T)和 Loktanella maricola JCM 14564(T)相邻，序列相似性为 98.5-98.2%。菌株 KMM 9530(T)的特征是水解能力较弱，不能同化大多数有机底物。主要的类异戊二烯醌是 Q-10，极性脂类由磷脂酰胆碱、磷脂酰甘油、双磷脂酰甘油、一种未知的磷脂、一种未知的胺脂和未知的脂类组成，主要脂肪酸是 C18:1ω7c。基于系统发育分析、DNA-DNA 杂交和表型特征，可以得出结论，新菌株 KMM 9530(T)代表 Loktanella 属中的一个新种，因此建议将其命名为 Loktanella maritima sp. nov.。该种的模式菌株为 KMM 9530(T)（=NRIC 0919(T)=JCM 19807(T)）。
