Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022 Vladivostok, Prospect 100 Let Vladivostoku, 159, Russia.
NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):148-154. doi: 10.1099/ijs.0.019513-0. Epub 2010 Feb 19.
Two Gram-negative, strictly aerobic, non-motile bacteria designated strains KMM 3851(T) and KMM 3842(T) were respectively isolated from a marine snail specimen (Umbonium costatum) and from surrounding sediments collected simultaneously from the shore of the Sea of Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains KMM 3851(T) and KMM 3842(T) were affiliated with the Roseobacter lineage of the class Alphaproteobacteria as a separate phylogenetic line adjacent to the members of the genus Thalassobacter. These novel isolates shared 98.5 % 16S rRNA gene sequence similarity and 15 % DNA-DNA relatedness. The major isoprenoid quinone of both strains was Q-10 and polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and aminophospholipids. In addition, strain KMM 3851(T) contained two unknown lipids, whereas strain KMM 3842(T) contained diphosphatidylglycerol. Fatty acid analysis revealed C(18 : 1)ω7c and C(16 : 0) as major components and small amounts of C(18 : 2). The DNA G+C contents were 60.4 mol% (KMM 3851(T)) and 58.5 mol% (KMM 3842(T)). Based on distinctive phenotypic characteristics, DNA-DNA hybridization data and phylogenetic distance, strains KMM 3851(T) and KMM 3842(T) should be classified as representatives of two novel species in a new genus, Litoreibacter gen. nov., with the type species Litoreibacter albidus sp. nov. (type strain KMM 3851(T) =NRIC 0773(T) =JCM 16493(T)) and a second species Litoreibacter janthinus sp. nov. (type strain KMM 3842(T) =NRIC 0772(T) =JCM 16492(T)).
两株革兰氏阴性、严格需氧、非运动细菌，分别命名为 KMM 3851(T)和 KMM 3842(T)，分别从海洋蜗牛标本（Umbonium costatum）和同时从日本海海岸采集的周围沉积物中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 KMM 3851(T)和 KMM 3842(T)属于α变形菌纲的玫瑰杆菌科，与属 Thalassobacter 的成员相邻，形成一个单独的进化枝。这两个新分离株的 16S rRNA 基因序列相似性为 98.5%，DNA-DNA 同源性为 15%。两个菌株的主要类异戊二烯醌均为 Q-10，极性脂类由磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和氨基磷脂组成。此外，菌株 KMM 3851(T)含有两种未知脂质，而菌株 KMM 3842(T)含有双磷脂酰甘油。脂肪酸分析表明，C(18:1)ω7c 和 C(16:0)为主要成分，少量含有 C(18:2)。DNA G+C 含量分别为 60.4 mol%（KMM 3851(T)）和 58.5 mol%（KMM 3842(T)）。基于独特的表型特征、DNA-DNA 杂交数据和系统发育距离，菌株 KMM 3851(T)和 KMM 3842(T)应被分类为一个新属中的两个新种的代表，即海生菌属（Litoreibacter），新种为白色海生菌（Litoreibacter albidus sp. nov.），模式株为 KMM 3851(T)=NRIC 0773(T)=JCM 16493(T)和第二个种为詹氏海生菌（Litoreibacter janthinus sp. nov.），模式株为 KMM 3842(T)=NRIC 0772(T)=JCM 16492(T)。
