Li Yunfeng, Luo En, Zhu Songsong, Li Jihua, Zhang Li, Hu Jing
1 State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu - PR China.
J Appl Biomater Funct Mater. 2015 Mar 18;13(1):28-34. doi: 10.5301/jabfm.5000168.
The objective of this study was to investigate cancellous bone response to strontium-doped hydroxyapatite (SrHA) in ovariectomized (OVX) rats.
Hydroxyapatite (HA) and 10%SrHA (HA with 10 mol% calcium substituted by strontium) implants were prepared and characterized by scanning electronic microscopy (SEM), energy dispersive microanalysis (EDX) and X-ray diffraction (XRD). Twelve weeks after bilateral ovariectomy, 20 rats randomly received HA or 10%SrHA implants in the right distal femur, with 10 animals in each group. Eight weeks after implantation, specimens were harvested and analyzed by micro-computed tomography (micro-CT) and histology.
Compared with HA, 10%SrHA raised the percentage bone volume by 42.6%, bone-to-implant contact by 47.1%, mean trabecular number by 27.3%, mean trabecular thickness by 31.5% and mean connectivity density by 37.4%, while it decreased mean trabecular separation by 20.1% in micro-CT evaluation. 10%SrHA also increased the bone area density by 47.6% in histological analysis.
With the HA implants as controls, the 10%SrHA implants were shown to increase bone density and bone-to-implant contact, and improve trabecular architecture in the vicinity of implant surfaces.
本研究的目的是调查去卵巢（OVX）大鼠的松质骨对掺锶羟基磷灰石（SrHA）的反应。
制备了羟基磷灰石（HA）和10% SrHA（10摩尔%钙被锶取代的HA）植入物，并通过扫描电子显微镜（SEM）、能量色散微分析（EDX）和X射线衍射（XRD）对其进行表征。双侧卵巢切除术后12周，20只大鼠随机在右股骨远端接受HA或10% SrHA植入物，每组10只动物。植入8周后，采集标本并通过微型计算机断层扫描（micro-CT）和组织学进行分析。
与HA相比，在微型计算机断层扫描评估中，10% SrHA使骨体积百分比提高了42.6%，骨与植入物接触率提高了47.1%，平均小梁数量提高了27.3%，平均小梁厚度提高了31.5%，平均连通性密度提高了37.4%，同时使平均小梁间距降低了20.1%。在组织学分析中，10% SrHA还使骨面积密度提高了47.6%。
以HA植入物作为对照，结果显示10% SrHA植入物可增加骨密度和骨与植入物的接触，并改善植入物表面附近的小梁结构。
