磷酸盐、三磷酸腺苷和二磷酸腺苷对铁从博来霉素铁（III）转移至脱铁转铁蛋白的刺激作用。

Stimulatory effect of phosphate, ATP and ADP on iron transfer from Fe(III)-bleomycin to apotransferrin.

Løvstad R A

Institute of Medical Biochemistry, University of Oslo, Norway.

Int J Biochem. 1989;21(5):555-9. doi: 10.1016/0020-711x(89)90137-7.

Abstract

The rate of ferric ion transfer from Fe(III)-bleomycin to apotransferrin was increased in the presence of orthophosphate, ATP and ADP, while AMP was without effect. 2. Ortho phosphate activation probably involves formation of a Fe(III)-bleomycin-phosphate complex. The optical absorption of Fe(III)-bleomycin at 450 nm is enhanced in the presence of phosphate. 3. ATP and ADP remove the ferric ion from the iron-drug complex; thus making the ferric ion readily available for uptake by apotransferrin. 4. Low concentrations of ATP, ADP and AMP, also enhance the 450 nm absorption of the iron-drug complex. Higher ATP and ADP concentrations reduce both the 450 and 384 nm absorption of Fe(III)-bleomycin.

摘要

在正磷酸盐、ATP和ADP存在的情况下，三价铁离子从博来霉素铁（III）转移至脱铁转铁蛋白的速率增加，而AMP则无此作用。2. 正磷酸盐激活可能涉及形成博来霉素铁（III）-磷酸盐复合物。在磷酸盐存在下，博来霉素铁（III）在450nm处的光吸收增强。3. ATP和ADP从铁-药物复合物中去除三价铁离子；从而使三价铁离子易于被脱铁转铁蛋白摄取。4. 低浓度的ATP、ADP和AMP也会增强铁-药物复合物在450nm处的吸收。较高浓度的ATP和ADP会降低博来霉素铁（III）在450nm和384nm处的吸收。