Ciotola Francesca, Albarella Sara, Scopino Giuseppe, Carpino Santo, Monaco Francesco, Peretti Vincenzo
Folia Biol (Krakow). 2014;62(1):23-8. doi: 10.3409/fb62_1.23.
Aneuploid cell percentages and frequencies of CAs and SCEs were investigated in 10 Calabrian pigs, 10 LW pigs and 19 Calabrian x LW crossbred pigs, in order to compare genome stability between an autochthonous pig breed and a highly selected one and to verify if genome stability of their progeny, as other phenotypic traits, are influenced by heterosis. The mean number of cells per animal with structural aberrations, excluding gaps, was 6.20 +/- 2.39, 4.90 +/- 2.02 and 4.52 +/- 3.34 in Calabrian, LW and crossbred pigs, respectively, while the mean number of total CAs without gaps was 0.14 +/- 0.38, 0.11 +/- 0.35 and 0.11 +/- 0.35, respectively. The mean number of SCEs was 7.30 +/- 3.24 in Calabrian pigs, 6.45 +/- 2.74 in LW pigs and 6.28 +/- 2.90 in the crossbred ones. Percentages of cells with aneuploidy were 7.30, 10.10 and 10.79 in Calabrian, LW and crossbred pigs, respectively. In particular, the Calabrian breed showed higher values compared to LW in each test, however, there were statistically significant differences only in the mean number of SCEs per cell (P<0.01). In addition, there is a positive effect of crossbreeding on baseline levels of genome stability in the crossbred group that shows in all tests, excluding gaps, mean values of cellular or chromosome damage similar to the LW group.
为了比较一个本地猪品种和一个高度选育猪品种之间的基因组稳定性，并验证它们后代的基因组稳定性是否像其他表型特征一样受杂种优势影响，对10头卡拉布里亚猪、10头长白猪和19头卡拉布里亚猪与长白猪的杂交猪进行了非整倍体细胞百分比以及染色体畸变（CAs）和姐妹染色单体交换（SCEs）频率的研究。卡拉布里亚猪、长白猪和杂交猪中，每只动物具有结构畸变（不包括裂隙）的细胞平均数分别为6.20±2.39、4.90±2.02和4.52±3.34，而无裂隙的总染色体畸变平均数分别为0.14±0.38、0.11±0.35和0.11±0.35。卡拉布里亚猪的SCEs平均数为7.30±3.24，长白猪为6.45±2.74，杂交猪为6.28±2.90。卡拉布里亚猪、长白猪和杂交猪的非整倍体细胞百分比分别为7.30、10.10和10.79。特别是，在每项检测中，卡拉布里亚品种的值均高于长白猪，然而，仅在每个细胞的SCEs平均数上存在统计学显著差异（P<0.01）。此外，杂交对杂交组基因组稳定性的基线水平有积极影响，在所有检测（不包括裂隙）中，该组细胞或染色体损伤的平均值与长白猪组相似。
