Hanthorn Christy J, Dewell Reneé D, Cooper Vickie L, Frana Timothy S, Plummer Paul J, Wang Chong, Dewell Grant A
Department of Veterinary and Diagnostic Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 2237 Lloyd Vet Med, Ames, IA 50011, USA.
BMC Vet Res. 2014 Apr 18;10:89. doi: 10.1186/1746-6148-10-89.
Bibersteinia trehalosi causes respiratory disease in ruminants particularly in wild and domestic sheep. Recently, there has been an increased number of B. trehalosi isolates obtained from diagnostic samples from bovine respiratory disease cases. This study evaluated the role of B. trehalosi in bovine respiratory disease using an intra-tracheal inoculation model in calves. Thirty six cross bred 2-3 month old dairy calves were inoculated intra-tracheally with either leukotoxin negative B. trehalosi, leukotoxin positive B. trehalosi isolate, Mannheimia haemolytica, a combination of leukotoxin negative B. trehalosi and M. haemolytica or negative control. Calves were euthanized and necropsy performed on day 10 of study.
B. trehalosi inoculated calves did not have increased lung involvement compared to control calves. Additionally, B. trehalosi was only cultured once from the lungs of inoculated calves at necropsy.
Based on these findings B. trehalosi may not be a primary pathogen of respiratory disease in cattle. Culture of B. trehalosi from diagnostic submissions should not be immediately identified as a primary cause of respiratory disease.
特氏比伯斯坦菌可引起反刍动物呼吸道疾病，尤其是野生和家养绵羊。最近，从牛呼吸道疾病病例的诊断样本中分离出的特氏比伯斯坦菌数量有所增加。本研究使用犊牛气管内接种模型评估了特氏比伯斯坦菌在牛呼吸道疾病中的作用。将36头2至3月龄的杂交奶牛犊气管内接种白细胞毒素阴性的特氏比伯斯坦菌、白细胞毒素阳性的特氏比伯斯坦菌分离株、溶血曼氏杆菌、白细胞毒素阴性的特氏比伯斯坦菌与溶血曼氏杆菌的组合或阴性对照。在研究第10天对犊牛实施安乐死并进行尸检。
与对照犊牛相比，接种特氏比伯斯坦菌的犊牛肺部受累情况并未增加。此外，尸检时仅在接种犊牛的肺中培养出一次特氏比伯斯坦菌。
基于这些发现，特氏比伯斯坦菌可能不是牛呼吸道疾病的主要病原体。从诊断样本中培养出特氏比伯斯坦菌不应立即认定为呼吸道疾病的主要病因。
