Kolomytkin O V, Mantsygin Iu A, Sviatukhina N V
Izv Akad Nauk SSSR Biol. 1989 May-Jun(3):394-400.
The monoclonal antibodies against asymmetric channel formed in the lipid bilayer of polyene antibiotic amphotericin B and cholesterol after addition of the antibiotic to the compartment from the cis side of the membrane were obtained. The effect of the antibodies on ion conductance of the channel depends on the distribution of cholesterol in the membrane. When cholesterol was present on both sides of the lipid bilayer, three antibody molecules bound to the channel from the trans side of the membrane, thus markedly increasing the lifetime of the open state of the channel. When cholesterol was present in the cis monolayer only, the antibodies, added to the trans compartment of the cell, reduced the membrane conduction.
制备了针对多烯抗生素两性霉素B与胆固醇在脂质双层中形成的不对称通道的单克隆抗体，该抗生素是从膜的顺式侧添加到隔室中的。抗体对通道离子电导的影响取决于胆固醇在膜中的分布。当脂质双层两侧均存在胆固醇时，三个抗体分子从膜的反式侧与通道结合，从而显著增加通道开放状态的持续时间。当仅顺式单层中存在胆固醇时，添加到细胞反式隔室中的抗体降低了膜传导。
