Pulsipher Abigail, Griffin Matthew E, Stone Shannon E, Brown Joshua M, Hsieh-Wilson Linda C
Division of Chemistry and Chemical Engineering, California Institute of Technology and Howard Hughes Medical Institute , 1200 East California Boulevard, Pasadena, California 91125, United States.
J Am Chem Soc. 2014 May 14;136(19):6794-7. doi: 10.1021/ja5005174. Epub 2014 Apr 30.
The ability to tailor plasma membranes with specific glycans may enable the control of signaling events that are critical for proper development and function. We report a method to modify cell surfaces with specific sulfated chondroitin sulfate (CS) glycosaminoglycans using chemically modified liposomes. Neurons engineered to display CS-E-enriched polysaccharides exhibited increased activation of neurotrophin-mediated signaling pathways and enhanced axonal growth. This approach provides a facile, general route to tailor cell membranes with biologically active glycans and demonstrates the potential to direct important cellular events through cell-surface glycan engineering.
用特定聚糖定制质膜的能力可能有助于控制对正常发育和功能至关重要的信号事件。我们报告了一种使用化学修饰脂质体用特定硫酸化硫酸软骨素（CS）糖胺聚糖修饰细胞表面的方法。经过工程改造以展示富含CS-E多糖的神经元表现出神经营养因子介导的信号通路激活增加和轴突生长增强。这种方法为用生物活性聚糖定制细胞膜提供了一种简便、通用的途径，并证明了通过细胞表面聚糖工程指导重要细胞事件的潜力。
