Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Department of Medical Microbiology and Medical Immunology, Rijnstate, Velp, The Netherlands.
J Hosp Infect. 2014 May;87(1):47-9. doi: 10.1016/j.jhin.2014.03.003. Epub 2014 Mar 26.
Rapid identification of patients colonized with carbapenemase-producing Enterobacteriaceae (CPE) is essential to prevent introduction and the spread of CPE in the hospital. This article presents the results of a new screening method to detect patients colonized with CPE within 24h after hospital admission. From high-risk patients rectal and throat swabs were collected and incubated overnight, after which DNA was isolated and tested for the most prevalent CPE genes (KPC, NDM, OXA-48, VIM and IMP) by a ligation-mediated real-time polymerase chain reaction. In 14 months 454 patients were screened; in six patients CPE were detected (carriage rate 1.3%).
快速鉴定产碳青霉烯酶肠杆菌科细菌（CPE）定植患者对于防止 CPE 在医院内的引入和传播至关重要。本文介绍了一种新的筛选方法，可在患者入院后 24 小时内检测出 CPE 定植患者。从高风险患者的直肠和咽喉拭子中采集样本并过夜孵育，然后提取 DNA，通过连接介导的实时聚合酶链反应检测最常见的 CPE 基因（KPC、NDM、OXA-48、VIM 和 IMP）。在 14 个月的时间里，对 454 名患者进行了筛查；在 6 名患者中检测到 CPE（携带率为 1.3%）。
