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[一种用于检测脑膜炎球菌抗原的改良毛细管乳胶凝集方法]

[A modified method of latex agglutination in capillaries for the detection of meningococcal antigens].

作者信息

Samsonova I M, Martynov Iu V, Koroleva I S

出版信息

Lab Delo. 1989(6):50-2.

PMID:2474706
Abstract

The suggested microvariant of the latex agglutination test in glass capillaries is not inferior to the traditional latex agglutination on the glass in sensitivity and specificity. The microvariant consists in the following: glass capillaries 0.8 mm in diameter 10 cm long are filled with the pathological material (3/5 of the tube length) and with the diagnostic latex preparation (1/5 of the tube length) so that no air hole is formed between them. The time of the reaction may be prolonged to 20-30 min without deteriorating its specificity. The results are recorded using a 4+ system, similarly as with the latex agglutination on the glass. The described modification appears to be useful for practical laboratories: it saves the latex preparation 4-fold and takes 2 times less pathologic material.

摘要

玻璃毛细管中乳胶凝集试验的建议微变体在敏感性和特异性方面并不逊色于传统的玻片乳胶凝集试验。该微变体如下:将直径0.8毫米、长10厘米的玻璃毛细管装入病理材料(占管长的3/5)和诊断用乳胶制剂(占管长的1/5),使它们之间不形成气孔。反应时间可延长至20 - 30分钟而不降低其特异性。结果采用4+系统记录,与玻片乳胶凝集试验相同。所描述的改进似乎对实际实验室有用:它可节省4倍的乳胶制剂用量,且所需病理材料减少一半。

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