Li Rongfeng, Yu Huahua, Xue Wei, Yue Yang, Liu Song, Xing Ronge, Li Pengcheng
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100039, China.
J Proteomics. 2014 Jun 25;106:17-29. doi: 10.1016/j.jprot.2014.04.011. Epub 2014 Apr 18.
Jellyfish Stomolophus meleagris is a very dangerous animal because of its strong toxicity. However, the composition of the venom is still unclear. Both proteomics and transcriptomics approaches were applied in present study to investigate the major components and their possible relationships to the sting. The proteomics of the venom from S. meleagris was conducted by tryptic digestion of the crude venom followed by RP-HPLC separation and MS/MS analysis of the tryptic peptides. The venom gland transcriptome was analyzed using a high-throughput Illumina sequencing platform HiSeq 2000 with de novo assembly. A total of 218 toxins were identified including C-type lectin, phospholipase A₂ (PLA₂), potassium channel inhibitor, protease inhibitor, metalloprotease, hemolysin and other toxins, most of which should be responsible for the sting. Among them, serine protease inhibitor, PLA₂, potassium channel inhibitor and metalloprotease are predominant, representing 28.44%, 21.56%, 16.06% and 15.14% of the identified venom proteins, respectively. Overall, our combined proteomics and transcriptomics approach provides a systematic overview of the toxins in the venom of jellyfish S. meleagris and it will be significant to understand the mechanism of the sting.
Jellyfish Stomolophus meleagris is a very dangerous animal because of its strong toxicity. It often bloomed in the coast of China in recent years and caused thousands of people stung and even deaths every year. However, the components which caused sting are still unknown yet. In addition, no study about the venomics of jellyfish S. meleagris has been reported. In the present study, both proteomics and transcriptomics approaches were applied to investigate the major components related to the sting. The result showed that major component included C-type lectin, phospholipase A₂, potassium channel inhibitor, protease inhibitor, metalloprotease, hemolysin and other toxins, which should be responsible for the effect of sting. This is the first research about the venomics of jellyfish S. meleagris. It will be significant to understand the mechanism of the biological effects and helpful to develop ways to deal with the sting.
海蜇（Stomolophus meleagris）是一种非常危险的动物，因其毒性很强。然而，其毒液的成分仍不清楚。在本研究中，蛋白质组学和转录组学方法都被用于研究主要成分及其与蜇刺可能的关系。通过对粗毒液进行胰蛋白酶消化，然后对胰蛋白酶肽进行反相高效液相色谱（RP-HPLC）分离和串联质谱（MS/MS）分析，来开展海蜇毒液的蛋白质组学研究。使用高通量Illumina测序平台HiSeq 2000对毒腺转录组进行分析，并进行从头组装。共鉴定出218种毒素，包括C型凝集素、磷脂酶A₂（PLA₂）、钾通道抑制剂、蛋白酶抑制剂、金属蛋白酶、溶血素和其他毒素，其中大多数应该与蜇刺有关。其中，丝氨酸蛋白酶抑制剂、PLA₂、钾通道抑制剂和金属蛋白酶占主导地位，分别占已鉴定毒液蛋白的28.44%、21.56%、16.06%和15.14%。总体而言，我们结合蛋白质组学和转录组学的方法提供了海蜇毒液中毒素的系统概述，这对于理解蜇刺机制具有重要意义。
海蜇是一种非常危险的动物，因其毒性很强。近年来它经常在中国沿海大量出现，每年导致数千人被蜇伤甚至死亡。然而，导致蜇伤的成分仍然未知。此外，尚未有关于海蜇毒液组学的研究报道。在本研究中，蛋白质组学和转录组学方法都被用于研究与蜇刺相关的主要成分。结果表明，主要成分包括C型凝集素、磷脂酶A₂、钾通道抑制剂、蛋白酶抑制剂、金属蛋白酶、溶血素和其他毒素，这些应该是造成蜇刺效应的原因。这是关于海蜇毒液组学的首次研究。这对于理解生物学效应机制具有重要意义，并有助于开发应对蜇刺的方法。
